荧光原位杂交技术在环境微生物生态学解析中的应用研究孙寓姣.pdfVIP

5 11 Vol.5, No.11 20 04 1 1 Techniques and Equipment for Environmental Pollution Control Nov .2 0 0 4 1, 2 1 1 孙寓姣 　王　勇 　黄　霞 (1., , 100084; 2., 150090) 　　, 。 。(FISH), 、 、、, 。, , 、 。、, 。, 。 　　　　　 Application of fluorescence in situ hybridization in analysis of environmental microbial ecology 1,2 1 1 Sun Yujiao 　Wang Yong 　Huang Xia (1.Environmental Simulation and Pollution Control State ey Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084; 2.Environmental Biotechnology Research Office, Harbin Industry University, Harbin 150090) Abstract　Recently, molecular biology methods are established and biology evaluation are carried out in environmental microbiology field in many countries.Some methods to analyse microbiological community without traditional culture-based methods are used extensively .Fluorescence in situ hybridization (FISH) technology can exactly reflect the natural colonial morphology of culturable and unculturable organisms.In FISH method, especial probes are used to hybridize with targeted rRNA in cells, and the exact identification of bacteria can be attained to genus and species level.Nitrifying bacterium probes, phosphate-removing bac- terium probes, and filamentous bacterium probes are often used in microbiology morphology, count, spatial distribution studies.FISH technology avoids the localization of bacterial traditional culture-based methods, and has agreat potential in environmental microbial ecology analysis. Key words　molecular biology;fluorescent in situ hybridization (FISH);oligonucleotide pro