草海流域优势树种碳酸酐酶与固碳释氧关系研究.pdfVIP

草海流域优势树种碳酸酐酶与固碳释氧关系研究 范怡雯，喻理飞 (贵州大学林学院，贵州贵阳 550025) 摘要：采用不同浓度 Ca 溶液对贵州草海流域优势树种华山松、滇杨、白栎一年生幼苗进行处理，测定不同处理 下碳酸酐酶活性、叶绿素含量、光合参数及固碳释氧量的变化，分析其对光合固碳能力影响。结果表明：(1) Ca 浓度对CA 活性呈低促高抑的趋势，阔叶树种滇杨和白栎CA 活性高于针叶树种华山松；(2) Ca 浓度的升高会抑 制PSII 反应中心的电子传递速率，植物通过增大叶片CA 活性，提升PSII 反应中心的原初光能转化效率，以增 强实际光合效率；(3)阔叶树种滇杨和白栎对Ca 最适浓度为100 mg/L，针叶树种华山松为50 mg/L，植物固碳释 氧能力与CA 活性具有相关性。说明喀斯特高钙适生植物能够利用生境特点，增强植物叶片碳酸酐酶活性，有效 促进其光合固碳能力，这对优化喀斯特岩溶地区植物固碳能力具有重要意义。 关键词：恢复生态学；高钙；优势种；草海流域；碳酸酐酶；固碳释氧 Relationship between Carbonic Anhydrase and Carbon Fixation and Oxygen Release of Dominant Species in Caohai Watershed FAN Yiwen, YU Lifei (College of Forestry, Guizhou University, Guiyang, 550025，China) Abstract: Three dominant species plant seeding in Cao Hai watershed, pinus armandi, populus yunnanensis and white oak, were used as materials to investigate the effects of different calcium contents on carbonic anhydrase (CA) activity, chlorophyll, fluorescence and Carbon Fixation and Oxygen Release. The influences of them on photosynthetic carbon sequestration ability were analyzed. The results show that: (1) The CA activity is promoted in low and suppressed in high concentration of Ca. The CA activities of broad-leaved species, populus yunnanensis and white oak, are stronger than pinus armandi. (2) Elevated Ca concentration will inhibit electron transport rate of PSII reaction center. The primary light energy conversion efficienty of PSII reaction center could be enhanced through the increased CA activity of plant leaf, and the actual photosynthetic efficiency can be enhanced as well. (3) The optimum Ca conce