NK细胞的制备和临床应用.pdfVIP

P A C T A R T I C L E Natural killer cells: a review of manufacturing and clinical utility Scott A. Koepsell, Jeffrey S. Miller, and David H. McKenna Jr_3724 404..410 atural killer (NK) cells are a heterogeneous ity.5 Other less-defined and smaller NK-cell subsets have group of immune cells that share several been described, and as new surface markers are discov- common identifying properties. Morphologi- ered, the definition and functional characteristics of NK Ncally, NK cells are large granular lymphocytes cells will be further refined. with abundant cytoplasm with azurophilic cytoplasmic NK cells were first discovered to recognize and lyse granules.1 Cell surface markers detected by flow cytometry cells lacking major histocompatibility complex (MHC) and immunohistochemistry are now standard to identify without prior sensitization.6,7 Later, NK cells were shown NK cells, which are defined by the presence of an isoform to lack germline T-cell receptor gene rearrangements and of the neural cell adhesion molecule, CD56, and the antigen-specific cell surface receptors.8 For these reasons, absence of CD3, the pan-T-cell marker.2 With these NK cells were traditionally classified as part of the innate phenotypic criteria, NK cells are present in significant immune system. However, newer research supports the numbers, comprising up to 29% of circulating lympho- notion that during NK-cell maturation, an education or cytes3 and 5% to 17% of mononuclear cells in hematolym- licensing event occurs that allows NK c