人肾上腺皮质癌裸鼠腹腔种植转移模型的建立及意义.pdfVIP

2011 Feb; 28( 1) JOURNA OF GUANGXI MEDICA UNIVERSITY 1 * 1 ( 530021) 6 :: 1 10 / m SW13 10 , 23 3 , 4 , Ki67 : 10 8 100% , 34 , , 100% , 4 2 , 1 , SW13 Ki67: , ; ; :R7366 :A : 1005930X( 2011) ESTABLISHMENT OF PERITONEAL METASTASIS MODEL OF ADRENOCORTICAL CAR CINOMA IN NUDE MICE AND ITS SIGNIFICANCE Zou Xinyu, uo Zuojie, Kuang Xiaocong, Qin Yingfen, iang Xinghuan, Wu ili, u Decheng. ( Department of Endocrinology, the First Affiliated Hospital of Guangxi Medical U niversity, Nanning 530021, China) Abstract Objective:To establish a peritoneal metastasis model of human adrenocortical carcinoma in nude mice and investigate its biological characteristics. Metho s: Human adrenocortical carcinoma SW13 cells 6 were cultured in vitro and a total of 110 / m cells were inoculated into the peritoneal cavity of ten nude mice. After 2 and 3 weeks; respectively, three nude mice were sacrificed randomly, the remaining nude th mice were sacrificed for autopsy and histology at 4 week. The neoplasia formation, tissue infiltration and metastasis in nude mice were observed dynamically. Tumor and related organs were investigated in patho logical morphology, and the expression of Ki67 was detected by immunohistochemistry. Results:The tumor take rate of all ten nude mice at the sites of injection was 100% by day 8. T hree and four weeks after inocu lation, there were gross peritoneal metastatic nodules in all mice. The mesentery was most significant, with the metastasis rate of 1