下一代测序技术在筛选转移性黑色素瘤中差异表达miRNA的应用研究.pdfVIP

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 33: 1117-1121, 2014 Identification of differentially expressed microRNAs in metastatic melanoma using next-generation sequencing technology 1 1 2 2 MIN QI , XIAOYUAN HUANG , LEI ZHOU and JIANGLIN ZHANG 1 2 Departments of Plastic Surgery and Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China Received November 14, 2013; Accepted February 20, 2014 DOI: 10.3892/ijmm.2014.1668 Abstract. In this study, we investigated differentially expressed cancer cells which differ from normal cells, eventually leading microRNAs (miRNAs or miRs) and their functions in metastatic to tumor recurrence, progression and ultimately, death (3). melanoma using next-generation sequencing technology. The The median survival of patients with metastatic melanoma is GSE36236 data set was downloaded from the Gene Expression 1 year (4). Thus, an improvement in therapy for metastatic Omnibus (GEO) database and 4 primary cutaneous melanoma melanoma is mandatory. samples (used as controls) and 3 metastatic melanoma samples A number of studies have revealed that microRNAs were selected from 31 samples for further analysis. Firstly, the (miRNAs or miRs) are associated with the metastasis of colon differentially expressed miRNAs were screened by limma cancer, breast cancer and lung cancer cells (5-7). Over the