碱性水饱和正丁醇萃取体系联合大孔树脂纯化茶皂素研究论文.pdfVIP

碱性水饱和正丁醇萃取体系联合大孔树脂纯化茶皂素研究 涂云飞，杨秀芳，孔俊豪 (中华全国供销合作总社杭州茶叶研究院，杭州 310016) 摘要：茶皂素为五环三萜类糖苷化合物，分子量较大，极性强，易溶于水，起泡性强。从茶籽饼中提取的茶皂素 粗品溶于水后色泽深。这类色素成分亦为极性较大的易溶于水的成分，从而给茶皂素的脱色纯化带来了困难。主 要通过碱性水饱和正丁醇对粗品茶皂素进行初步脱色与提纯，再利用筛选的大孔树脂对萃取相中的皂素进行进一 步的脱色，以制备色泽浅的茶皂素产品。结果表明，茶皂素在中强碱的水饱和正丁醇中的溶解度大于未加碱的萃 取体系，同时色素得到大幅度去除。另外，以D280 树脂进行后续脱色处理后，产品的纯度达到9 1 % 。结论：两 种技术手段结合纯化茶皂素可以达到预期的目的。 关键词：茶学；皂素；碱性水饱和正丁醇；大孔树脂1 Combination of Both Basic Water Saturated Butanol Extraction System and Macroporous Resin for Purication of Tea-Saponin TU Yunfei, YANG Xiufang, KONG Junhao (Hangzhou Tea Research Institute, All China Federation of Supply and Marketing Cooperatives, Hangzhou 310016, China) Abstract: Tea-saponins as the glycoside of Pent-acyclic Triterpenoid, which is extracted from camellia seeds, have a high molecule weight, strong polar, and easily water solubility characteristics. They are not only effective in decontamination, foaming, emulsification, decentralization and saturation, but also with function of diminishing inflammation, easing pain and resisting epiphyte. However, for the dark brown pigments of crude extraction from tea-cake were hard to remove using traditional methods, the saponins in the field of food, drink, hair care products and high-end Daily Health products are strongly restricted. In this paper, we employed basic water saturated butanol extraction system combination with macroporous resin for purication of tea-saponins. The results showed that tea-saponins were preferred to dissolve in the upper phase of basic water saturated butanol extraction system under the water solution containing moderate alkali. And the D280 was the optimum macroporous resin for removing the pigments after the extraction of basic water saturated