文献_2009-A distribution of two SNPs in exon 10 of the FSHR gene among the women with a diminished ovarian reserve in Ukraine.pdfVIP

J Assist Reprod Genet (2009) 26:29–34 DOI 10.1007/s10815-008-9279-1 GENETICS A distribution of two SNPs in exon 10 of the FSHR gene among the women with a diminished ovarian reserve in Ukraine Ganna Livshyts Svetlana Podlesnaja Sergey Kravchenko Iryna Sudoma Ludmila Livshits Received: 4 September 2008 /Accepted: 5 November 2008 /Published online: 25 November 2008 # Springer Science + Business Media, LLC 2008 Abstract Conclusions Our data shows the prevalence of the Ala307- Purpose To evaluate the association between phenotype Ser680/ Ala307-Ser680 genotype in the both groups of and follicle stimulating hormone receptor (FSHR) genotype patients. The finding should have impact on the delineation in women with ovarian dysfunction and patients with “poor of stimulation protocols. response” to gonadotropin stimulation of ovulation. Methods FSHR gene SNPs were analyzed by PCR and RFLP. Keywords FSH receptor . DNA polymorphism . “Poor responders” (ovarian dysfunction) group and “good Ovarian reserve . FSH stimulation responders” group constituted the study group. Normo- ovulatory women who gave birth to naturally conceived children formed control groups: under 35 years of age (control Introduction I) and over 35 years of age (control II). Results The frequency of Ala307-Ser680/Ala307-Ser680 Follicle stimulating hormone (FSH) is one of the pituitary genotype was significantly more prevalent in the ovarian glycoproteins that plays an important role during folliculo- dysfunction group (26%) compared to the control I (7.7%) genesis by triggering the maturation of follicles, i.e. the (P 0.001) and a “good responders” group (12.5%) (P proliferation of granulosa cells, and induces synthesis of the 0.05); and in