黑曲霉利用五倍子生料固体发酵产单宁酶优化发酵条件的研究.docVIP

黑曲霉利用五倍子生料固体发酵产单宁酶优化发酵条件研究 摘 要:单宁酶全称是单宁酯酰水解酶(Tannase, E,它可以水解没食子酸单宁中的酯键和缩酚羧键,生成没食子酸和葡萄糖。主要来源于微生物,存在于细胞内和细胞外,目前研究得较多较为深入的是青霉和黑曲霉。本实验对五倍子生料固体发酵产单宁酶进行了研究,发酵条件的单因素优化后得到:初始水分含量80%,接种量1%, 20%五倍子,温度30e为最佳产酶条件。在此基础上,利用正交实验对显著影响产单宁酶的培养基及培养条件因素进行优化,得到固体发酵黑曲霉诱变菌株最佳产单宁酶条件为:湿度80%、接种量1%、发酵温度33e、五倍子含量15%。其中温度对黑曲霉产单宁酶酶活性的影响是显著的,并测得在此最佳发酵条件下产单宁酶活性达57132U/gds。 关键词:单宁酶;黑曲霉;固体发酵;优化 Optim ization of the production of tannase byAspergillus nigersolid-state fermentation using gallnut SHIRu-i li1,2, QIU Shu-yi1,2, LIYang-zhen1,2, ZHANG YI-m ing1,3 (1. Guizhou ProvinceKeyLab ofFermentation Engineering and Biophamacy, Guiyang 550003; 2. ChemicalEngineeringCollege, GuizhouUniversity, Guiyang 550003) Abstract:The systematic name ofTannase is tannin acylhydrolase (Tannase, the EC; it can hydrolyses the ester and depside bonds of gallotannis and gallic acid esters to form glucose and gallic acid.Tannasewasmainly produced bymicrobiology, existing inside and outside of the cel.l Penicillium andAspergillusNigerwere mostly stud- ied. This experimentwas focused on optimizing fermentation conditions byAspergillusNigerin solid-state fermentation using gall nuts as substrate. The initialmoisturewas 80%, the inoculum 1% and the 30 C was the best conditions by the single factor optimization experiment. Furthermore, orthogonal optimization of themedium and the culturewas per- formed and the best conditions ofproducing tannase by solid fermentationwere: the initialmoisture content80%, the inoculum 1%, the temperature 33eand the gallnuts15%. Among them, the temperature had a remarkable effecton Tannaseactivities. The activity ofTannasewas up to 57132U/gds under the above condition. Key words:Tannase; AspergillusNiger;solid fermentation; optimization 收稿日期:2009-02-25 基金项目:贵州省科学技术基金,黔科合J字[2008] 2091号。 作者简介:石瑞丽(1985-),女,河南许昌人,在读硕士研究生,研究方向为发酵工艺。中国食品添加剂 China FoodAdditives试验研究 108 单宁酶(Tannase, EC是一种水 解酶,全称单宁酯酰水解酶,能水解没食子酸单 宁中的酯醚和缩酚酸键生成没食子酸和其他化合 物,也能水解没食子酸甲酯类物质[1]。几十年来 科学家发现,能够产生单宁酶