基础研究山奈酚诱导人食管鳞癌Eca-109细胞凋亡及其机制.pdfVIP

南方医科大学学报（J South Med Univ ） 第31 卷 ·1440 · 南方医科大学学报（J South Med Univ ） 2011;31(8) ·基础研究· 山奈酚诱导人食管鳞癌Eca- 109细胞凋亡及其机制 李瑞君，梅家转，刘桂举 郑州人民医院肿瘤内科，河南 郑州 450003 摘要：目的研究山奈酚对人食管鳞癌Eca- 109细胞增殖和凋亡的影响，并探讨其机制。方法山奈酚体外作用于Eca- 109 细胞后，用MTT 法检测细胞增殖抑制作用；TUNEL 染色测定细胞凋亡率；RT-PCR 检测Bax、Bcl-2 基因表达变化情况；分 光光度法测定Caspase-3 和Caspase-9 活性。结果山奈酚显著抑制Eca- 109 细胞增殖（P0.01），呈剂量依赖关系。 TUNEL 染色结果表明山奈酚诱导Eca- 109细胞发生凋亡，RT-PCR 结果显示山奈酚作用后，肿瘤细胞Bax 基因表达上调， Bcl-2 基因表达下调，同时，Caspase-3 和Caspase-9 活性明显升高（P0.01）。结论山奈酚能抑制Eca- 109细胞增殖，诱导 细胞凋亡。山奈酚可能通过线粒体途径诱导Eca- 109细胞凋亡。 关键词：山奈酚；食管癌；细胞凋亡；Eca- 109细胞株 中图分类号：R735.1 文献标志码：A 文章编号：1673-4254（2011）08-1440-03 Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism LI Rui-jun, MEI Jia-zhuan, LIU Gui-ju Department of Medical Oncology, Zhengzhou People ’s Hospital, Zhengzhou 450003, China Abstract: Objective To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism. Method The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay. Results Kaempferol significantly inhibited Eca-109 cell proliferation (P0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caus