中药药物血清诱导骨髓间充质干细胞转化为肝细胞的研究.docVIP

  中药药物血清诱导骨髓间充质干细胞转化 为肝细胞的研究#  韩海啸，王志斌，陈润花  * 5 10 15 20 25 30 （北京中医药大学东方医院，北京，100078） 摘要：目的：探讨骨髓间充质干细胞(MSC s) 体外诱导其分化为肝细胞的可行性及中药调肝 益气通络方的有效干预性。方法：分离、纯化并鉴定大鼠骨髓 MSC,灌胃纤维化模型大鼠以 调肝益气通络方后提取药物血清, 用含药血清，对照肝细胞生长因子（Hepatocyte Growth Factor ,HGF）诱导 MSC 向肝细胞的分化若干天后。观察各组细胞形态变化, MTT 法测定细 胞增殖,免疫组化检测甲胎蛋白（Alpha Fetoprotein ,AFP）与白蛋白（Albumin ,ALB）表 达。 结果：1、各浓度含药血清在加入 1，7，14 天后，其细胞数量均显著多于常规培养组。 2、含药血清诱导 MSC 分化 7,14 天后，高剂量组 AFP 与 ALB 的阳性细胞率均显著高于 HGF 组。结论：中药调肝益气通络方具有诱导 MSC 分化为肝细胞的能力。 关键词：中药药物血清；间充质干细胞；肝细胞；诱导分化； 中图分类号：R979.1 Effect of Chinese medicine drug serum on the differ entiation of bone mar row mesenchymal stem cells into hepatocytes HanHaixiao, WangZhibin, ChenRunhua (Oriental Hospital of Beijing University of Chinese Medicine,beijing,100078) Abstract: AIM: To investigate the value of bone marrow mesenchymal stem cells (MSC s) in vitro differentiation of liver cells ， and The effective intervention of Yiqi Tongluo Decoction. Methods:Rat bonem arrow M SCs were isolated, purified and identified; normal and cholestaticsera of rats were prepared;Yiqi Tongluo Decoction orally fibrosis in rats after the extraction of drug serum; MSC drug-containing serum-induced liver cell differentiation 1 ，7 ，14 days; morphology was observed by immunohistochemistry of liver cells iconic protein alpha-fetoprotein (AFP), albumin (ALB), is detected.MTT assay cell growth curve,Results: 1, the concentration of serum containing 1,7,14 days, the number of cells than the conventional training group increased with the dosage, the time is proportional to.2. Serum induced differentiation of MSC positive cells after 7,14 days, AFP 、 ALB of high dose group were significantly higher than in HGF group. Conclusion: YiQi Tong Luo Fang has the ability to induce MSC differentiation into liver cells Key words: Chinese medicine drug serum ； bone mar row mesenchymal stem cells ； hepatocytes.；Differentiation； 35 0 前言 近年来的研究表明, 骨髓间充质干细胞(M esenchymal stemcel, MSCs)在一定条件下能 分化为肝细胞[ 1] , 这为临床上解决肝细胞移植、生物人工肝等的肝源短缺问题提供了新