犬心肌缺血再灌注损伤早期氧化应激指标的变化.docVIP

  犬心肌缺血再灌注损伤早期氧化应激指标 的变化# 1 2 1 1 1  5  （1. 中国医科大学附属第一医院老年病心内科 沈阳 110001； 2. 北京军区北戴河疗养院医务部 秦皇岛市 066100） 摘要：【目的】探讨犬心肌缺血再灌注损伤后早期，氧化应激指标超氧化物歧化酶（SOD） 的活力，以及丙二醛（MDA）含量在血清中的变化，研究其随缺血再灌注损伤时间的变化规 律以及在早期心肌缺血再灌注损伤中发挥的作用。【方法】健康杂种犬 14 只，随机分为假 10 15 20 25 30 35 40 手术组和缺血再灌注组(每组 7 只)。采用经股动脉冠状动脉球囊闭塞法建立犬心肌缺血再灌 注模型；检测犬血清中不同时间点的 SOD 和 MDA 的水平。【结果】与假手术组比较，缺血再 灌注组血清 SOD 活力和 MDA 含量，在再灌注 30min 后，出现明显变化,差异在再灌注 90min 后最显著。随后两者变化趋势与假手术组的差异逐渐减小，但恢复不到假手术组的水平。血 清中 SOD 活力和 MDA 含量的变化成负相关(r=-0.89,P 0.01)。【结论】血清中 SOD 活力和 MDA 含量与心肌缺血再灌注损伤严重程度有关。检测血清中 SOD 和 MDA 水平将对缺血再灌注 疾病的早期诊断与预后产生帮助。 关键词：心血管病学；心肌缺血再灌注损伤；氧化应激 中图分类号：R-331 Indicators of oxidative stress changes in myocardial ischemia-reperfusion injury in dog REN Lina1, SUN Yang2, LI Yuze1, CHENG Ling1, QI Guoxian1 (1. Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang, 110001; 2. Beidaihe Sanatorium Medical Department of the Beijing Military Region, Qinhuangdao, 066100) Abstract: Objectives: To discuss the changes of oxidative stress indicators superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum with time after early myocardial ischemia-reperfusion in dogs. Methods: 14 mongrel dogs were randomly divided into sham group and ischemia-reperfusion (I/R) groups (n=7). The model of canine myocardial ischemia-reperfusion was established via the femoral artery using coronary balloon occlusion. Serum SOD activity and MDA content were detect. Results: Compared with the sham group, I/R group activity of SOD and MDA after reperfusion 30min, significant changes over time, the most significant difference was after reperfusion 90min. Then the difference trend between the sham group gradually decreased, but the level of recovery than the sham group. Serum SOD activity and MDA content was negatively correlated (r=-0.89, P 0.01). Conclusions: Serum SOD activity and MDA content reflect the severity of myocardial ischemia-reperfusion injury. Serum levels of SOD