复方浙贝颗粒联合阿霉素对AML-KG-1a移植瘤PI3K_AKT信号通路影响.docVIP

  复方浙贝颗粒联合阿霉素对 AML-KG-1a 移 植瘤 PI3K/AKT 信号通路影响# 1 1,2** 5 10 15 20 25 30 35 40 （1. 北京中医药大学东直门医院,北京 100700； 2. 中医内科学教育部和北京市重点实验室,北京 100700） 摘要：目的：通过免疫组化的方法探讨复方浙贝颗粒联合阿霉素对 KG-1a 细胞移植瘤 PI3K/AKT 信号通路的影响。方法：将 KG-1a 细胞接种 BALB/c-nu 裸小鼠腋前皮下构建移植 瘤动物模型，给予复方浙贝颗粒灌胃联合阿霉素腹腔注射，实验结束后处死小鼠，剥离肿瘤， 将瘤块组织制成切片，通过免疫组化结合 Image Pr0Plus 6.0 免疫组化彩色图像分析，观察 复方浙贝颗粒联合阿霉素对 KG-1a 移植瘤细胞 PI3K/AKT 相关蛋白表达的影响。结果：与阿 霉素组比，高剂量 CZBG 可以明显抑制 mTOR 蛋白的表达（P＜0.05），CZBG 各剂量组均可以 抑制 NF-κB 蛋白的表达（P＜0.05）。结论：CZBG 可以协同阿霉素下调 mTOR 及 NF-κB 的 表达。 关键词：复方浙贝颗粒；KG-1a 细胞系；PI3K/AKT 信号通路; 中图分类号：请查阅《中国图书馆分类法》 The Effect of Compound Zhe Bei Granule(CZBG) combined with Doxorubicin on the Expression of mTOR,PI3K,NF-κB and PTEN in KG-1a cell line tumor xenografts in Mice. Zhang yu1, Chen xinyi1,2 (1. Dongzhimen Hospital Beijing University of Chinese Medicine,Beijing 100700; 2. Key Labortary of Beijing and Ministry of Education of TCM internal Medicine,Beijing 100700) Abstract: Objective：To investigate the effect of Compound Zhe Bei granule(CZBG)combined with doxorubicin on the expression of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line transplantation tumors in Mice．Methods：Subcutaneous tumor xenograft model is established by injecting the acute myeloid leukemia cell line KG-1a in the axillary flank of BALB/c-nu nude mice．Drug-combination of CZBG intragastric administration and doxorubicin intraperitoneal injection (i.p.) is given to the BA LB/c-nu nude mice．The tumor xenografts are made into slice after the dissection, and the expression of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line tumor xenografts are detected by immunohistochemical technique. The optical density(OD) of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line tumor xenografts are measured by Image PmPlus6.0．Results: Compared with ADM group , high-doseage CZBG can inhibit the mTOR expression obviously(P＜ 0.05) ， mid-doseage and high-doseage CZBG can both inhibit the NF-κB expression（P＜0.05) ．Conclusion: CZBG can collaborate with doxorubicin to reduce the expression of mTOR