绿色荧光蛋白介绍.pptVIP

绿色荧光蛋白（green fluorescence protein; GFP） Sun qionglin definition The?green fluorescent protein?(GFP) is a protein??composed of 238 amino acid??residues that exhibits bright green fluorescence? when exposed to light in the blue to?ultraviolet?range.? Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish??. The beautiful jellyfish Nobel Laureate In?cell?and?molecular biology, the GFP?gene?is frequently used as a protein tagging. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a?viral vector, or cell transformation. And then using a fluorescence microscope to detect it * * 氨基酸残基 水母 Osamu Shimomura(下村修)、 Martin Chalfie(马丁·查尔菲)和Roger Y. Tsien(钱永健) The three scientists won that year (2008) of the Nobel Prize in chemistry for discovering and transforming green fluorescent protein 蛋白标签 Application 线粒体中表达的GFP ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? 科学使用绿色荧光蛋白跟踪大脑细胞的活动 With green fluorescent protein markers of the corresponding cell components (tubulin, microfilament, middle fiber) , we can observe cytoskeleton, which deepen our understanding of cellular support system. 用绿色荧光蛋白标记相应的细胞成分（微管蛋白、微丝、中间纤维）可以观察到组成细胞骨架的微管和微丝，可以加深我们对细胞支持系统的了解。 *