天然化合物TUE-1和TUE-2体外抗MRSA生物被膜的研究.docVIP

 Anti-biofilm Activity of Resveratrol and Ursolic Acid Against MRSA Pathogens in Vitro# TAN Xiaojuan1, YANG Dongting1, QIN Nan2, HUANG Zongjun1, JIA Aiqun1** 5 10 15 20 (1. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, NanJing 210014; 2. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University, HangZhou 310003) Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is one of main pathogens of medical device-related infections due to its adhesion and biofilm-forming capacity on the organ surfaces of host. Traditional antibiotic therapy is only able to eliminate planktonic cells, leaving the sessile forms to propagate within the biofilm and continue to disseminate when therapy is terminated. Recently, some quorum sensing inhibitors (QSIs) could be an alternative tool to suppress the emergence and the spread of these organisms by inhibiting or removing the bacterial biofilm. Here, we found that two isolated natural compounds in our group, TUE-1 and TUE-2, could block biofilm formation of MRSA. Although the inhibiting effects of ursolic acid and resveratrol on planktonic bacteria have been investigated in a few studies, their capacities of inhibiting biofilm formation or removing mature biofilm of MRSA have not been reported to date. In this study, in vitro viability assays of biofilm were performed that TUE-2 at 30μg/ml and TUE-1 at 100μg/ml inhibited biofilm formation of MRSA with 18h incubation time, and TUE-1 at 150μg/ml may partly remove established biofilm. Although the mechanisms of inhibiting biofilm formation and disrupting mature biofilm by TUE-1 and TUE-2 are not fully understood, data investigated here indicated a potential application for TUE-1 and TUE-2 as adjuvant therapeutic agents for the prevention of biofilm-related infection. Keywords: MRSA; Biofilm; TUE-1; TUE-2; Natural products 25 0 Introduction In recent years, Staphy