NG2参与炎症反应介导的肾小球系膜细胞增殖.docVIP

 NG2 参与炎症反应介导的肾小球系膜细胞 增殖# 史秀岩，熊京** 5 10 15 20 （华中科技大学同济医学院附属协和医院肾内科，武汉 430022） 摘要：目的 研究 NG2 在肾小球炎症因子产生与系膜细胞增殖中的作用，并探讨其作用的分 子机制。 方法 (1) 培养大鼠肾小球系膜细胞（RMC），LPS 刺激、Dexamethasone 干预及 siRNA 转染，免疫荧光、PCR、实时定量 PCR 和 Elisa 法检测 NG2、TNF-α和 IL-1β的表 达，MTT 法检测 RMC 增殖。(2) 新生乳鼠，LPS 刺激，免疫组化、实时定量 PCR 和 Elisa 法检测 NG2、TNF-α和 IL-1β的表达。 结果 (1)大鼠肾小球系膜细胞表达 NG2，LPS 刺激 后肾小球系膜细胞 NG2 表达显著增加，炎症因子 TNF-α和 IL-1β表达上调，且系膜细胞增 殖作用增强。(2) Dexamethasone 可抑制 NG2 和 TNF-α、IL-1β的上调，以及 RMC 增殖水 平的上调。(3) siRNA 下调 NG2 表达后，LPS 刺激引起的 TNF-α、IL-1β的表达下调，RMC 增殖水平下调。 结论 NG2 参与肾小球炎症反应的激活，介导炎症因子在肾小球系膜细胞 的表达和释放，并通过炎症因子促进系膜细胞的增殖。 关键词：NG2 蛋白多糖; 炎症因子；肾小球系膜细胞 中图分类号：S941.42+7 NG2 involved in the inflammation mediated mesangial cell proliferation SHI Xiuyan, XIONG Jing (Department of Nephrology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology, Wuhan 430022) Abstract: Objective To explore the role of NG2 proteoglycan in the proliferation of mesangial 25 30 35 40 cells induced by inflammation. Method Cultured rat mesangial cells (RMC) was stimulated by LPS, and the expression of NG2, TNF-α , IL-1β was measured by real-time PCR, western blot and immunofluorescence, and cell proliferation assay was detected by MTT. Dexamethasome and NG2 siRNA was used to treat the cells with LPS stimulation and observe the changes of above indexes. In vivo experiment, newborn mice were injected by LPS, and the expression of NG2 and inflammation factors was observed. Results The expression of NG2, TNF-α and IL-1β increased in RMC and the proliferation of RMC increased after LPS stimulation. Dexamethasone and NG2 siRNA treatment can ameliorate the produceion of inflammation factors and the proliferatin of RMC. Conclusion NG2 proteoglycan may be involved in the proliferation of mesangial cells induced by inflammation. Key words: NG2 proteoglycan; inflammation factor; mesangial cells 0 引言 NG2 是一种重要的硫酸软骨素蛋白多糖大分子，由 1 条相对分子质量为 300000 的核 心蛋白和 1 条硫酸软骨素链组成[1]。NG2 首先在中枢神经系统的少突胶质前体细胞（OPCs） 和周细胞中发现，在调节神经系统的发育如神经细胞迁移和轴突