补髓生血颗粒对再生障碍性贫血患者骨髓细胞内Ca2+浓度水平的影响及不同血液疾病间差别的观察.docVIP

 补髓生血颗粒对再生障碍性贫血患者骨髓 细胞内 Ca2+浓度水平的影响及不同血液 疾病间差别的观察# 5 10 15 20 25 30 35 40 罗正凯1，王金环2，雍延礼2，孙伟正2，孙凤2* （1. 黑龙江中医药大学临床医学院，哈尔滨 150001； 2. 黑龙江中医药大学附属第一医院，哈尔滨 150001） 摘要：目的：通过观察慢性再障患者骨髓单个核细胞内细胞钙离子浓度的变化, 探讨补髓生 血颗粒对慢性再障的治疗机制。观察不同血液疾病骨髓单个核细胞内细胞钙离子浓度的变化 以期为血液疾病的诊断和治疗提供新的思路。方法：提取慢性再障患者骨髓单个核细胞，使 用 fluo3 AM 进行荧光染色，使用流式细胞仪进行细胞内钙离子浓度的检测。同时提取慢性 再障、骨髓增生异常综合征、特发性血小板减少性紫癜、缺铁性贫血患者及正常志愿者骨髓 2+ 2+ 2+ 优于对照组，但仍高于正常组。结论：1、再生障碍性贫血患者骨髓单个核细胞内钙离子浓 2+ 放，可能是其治疗 CAA 的作用机制之一。2、MDS、CAA、CITP、IDA 不同血液疾病之间钙离 子浓度存在明显差异，可能与疾病恶性程度及发病机理相关。 2+ 中图分类号：R555 THE IMPACT OF Ca2+ CONCENTRATION IN BONE MARROW MONONUCLEAR CELLS WITH BUSUISHENGXUE GRANULE IN CHRONIC APLASTIC ANEMIA PATIENTS AND THE OBSERVATION OF IT IN DIFFERENT BLOOD DISEASES Luo Zhengkai1, Wang Jinhuan2, Yong Yanli2, Sun Weizheng2, Sun Feng2 (1. Clinic College of Medicine, Heilongjiang University of Chinese Medicine, Harbin 150001; 2. The First Affiliated Hospital Heilongjiang University of Chinese Medicine, Harbin 150001) 2+ cells(BMMC) in chronic aplastic anemia (CAA) , exploring the mechanism of BUSUISHEGNXUE granule on CAA. To explore diagnosis and treatment’s new ideas by observing the Ca2+ concentration in different blood dieases.Method: Extraction separation the CAA patients’ BMMC. Use fluo3 AM staining using flow cytometry detection of intracellular calcium concentration. Detect the Ca2+ concentration in CAA, Myelodysplastic Syndrome(MDS), Idiopathic Thrombocytopenic Purpura(ITP), Iron-Deficiency Anemia(IDA).Result: CAA patients’ BMMC intracellular Ca2+ concentrations were significantly higher than the normal group.After Busuishengxue particle treatment, patients in each group of intracellular Ca2+ concentration decline, Busuishengxue particle group than the control group, but still higher than the normal group.Conclusion: The concentration of BMMC intracellular calcium rise is an important symbol of CAA disease situation. The Busuishengxue particles can regulate Ca2+ release in BMMC , may be one