高眼压大鼠视网膜中NgR1的表达增加.docVIP

 高眼压大鼠视网膜中 NgR1 的表达增加# 苏毅华1，付清玲2** （1. 中山大学附属第一医院眼科，广州 510080； 5 10 15 20 25 30 35 40 2. 中山大学附属第一医院耳鼻咽喉科医院，广州 510080） 摘要：目的：NgR1 是中枢神经系统的一种可以抑制轴索再生的分子，最近发现其也可以影 响细胞的存活，本实验旨在研究大鼠高眼压损伤后视网膜中 NgR1 的变化。方法：选取 10 只成年雌性健康 SD 大鼠，随机分为实验组和对照组各 5 只，实验组经电凝右眼的三条巩膜 外静脉以诱导大鼠的高眼压，对照组小鼠右眼行假手术，只打开相应巩膜而对巩膜外静肪不 做处理，术后每三天一次测量眼压。术后 14 天处死大鼠取眼球行固定、包埋、视网膜切片、 免疫组织化学法行 NgR1 染色，观察视网膜中 NgR1 的表达。结果 对照组视网膜免疫组化验 证 NgR1 蛋白主要表现在节细胞层和神经纤维层，而诱导高眼压后 NgR1 的表达增高。结论 大 鼠高眼压损伤后视网膜中 NgR1 的表达增加，可能参与了视网膜受损后节细胞的死亡，如果 针对性的对其干预将可能有效的保护受损的视网膜节细胞。 关键词：眼科；高眼压；视网膜；NgR1 中图分类号：R775.3 The expression of NgR1 increased after the induction of ocular hypertension in rat. SU Yihua1, FU Qingling2 (1. Department of ophthalmology, GuangZhou 510080; 2. Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, GuangZhou 510080) Abstract: Objective To investigate the expression of NgR1 after the induction of ocular hypertension in rats. Method 10 healthy adult female SD rats were randomly grouped to experimental group and control group. The experimental group received an induction of intraocular pressure on their right eyes by cautery of 3 episcleral veins. The right eyes in the control group were received sham-operation. Rats were killed 14 days after the operation. NgR1 was immunohistochemically stained in the retinas. Result NgR1 positive staining was visible mainly in the ganglion cell layer (GCL) and the nerve fiber layer (NFL) in the normal retina. Normal RGCs expressed moderate staining for NgR1 but stronger immunoreactivity in the density of labeling for NgR1 occurred in RGCs 2 weeks after the induction of hypertension. Conclusion These results suggest the high expression of NgR1 in the retina in glaucoma and appropriate NgR1 antagonism may delay the progression of the disease. Keywords: ophthalmology; Ocular hypertension; Retina; NgR1 0 引言 青光眼是一类以视网膜节细胞（retinal ganglion cell, 视网膜节细胞）及其轴突的渐近性 退变为特征的神经退行性病变(1)。青光眼作为当前不可逆性致盲眼病的第二位原因,对社会 和家庭造成的损失较大。青光眼所引起的视功能障碍是不可逆的，所以对青光眼这种眼病应 早期发现，早期治疗。 长久以来