亚洲栽培稻与非洲栽培稻杂交种间杂种花粉不育基因的精细定位.docVIP

 n 亚洲栽培稻与非洲栽培稻杂交种间杂种花 粉不育基因的精细定位# 赵志刚，汪勇，王超龙，万建民** 5 10 15 20 25 30 35 40 （南京农业大学作物遗传与种质创新国家重点实验室，南京 210095） 摘要：栽培稻分为亚洲栽培稻（Oryza sativa L., 2n =2x= 24）和非洲栽培稻（O. glaberrima Steud, 2n = 2x= 24）两个栽培种，非洲栽培稻种资源中广泛存在着各种抗病虫、耐盐碱、 抗旱、耐淹、耐高温等相关性状的优良基因，但由于栽培稻种间杂种的高度不育，严重限制 了非洲栽培稻有利基因向亚洲栽培稻转移及栽培稻种间杂种优势的利用。为阐明亚洲栽培稻 与非洲栽培稻种间杂种不育的机理和进一步利用种间杂种优势，本研究以滇粳优 1 号（DJY1） 为受体亲本，非洲栽培稻 IRGC102295 为供体亲本，构建了一个近等基因系 NIL，滇粳优 1 号与 NIL 杂种 F1 花粉表现典型的半不育，败育类型为染败，受一对杂合基因座位 S37 控制。 利用 743 株滇粳优 1 号与 NIL 杂交 F2 群体进行连锁分析，初步将 S37 限定在第 12 染色体短 臂末端的两标记 NJ5 和 G4 之间，随后利用扩大的 18014 株 F2 群体，最终将 S37 精细定位到 标记 HP14 和 G21 之间，物理距离为 73 kb。为最终打破非洲栽培稻和亚洲栽培稻之间的生 殖隔离，筛选出具有优良性状的水稻新种质创造了条件。 关键词：花粉不育；分子标记；精细定位；水稻 中图分类号：S511 Fine mapping of hybrid pollen sterility gene between Oryza saliva L. and Oryza glaberrima S. Zhao Zhigang, Wang Yong, Wang Chaolong, Wan Jianmin (State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Center, Nanjing Agricultural University, NanJing 210095) Abstract: IWe developed an NIL via repeated backcrossing and molecular marker-assisted selection (MAS), where the japonica variety Dianjingyou 1 was used as the receptor parent and O. glaberrima Steud variety IRGC102203 as the donor parent. F1 pollen fertility of NIL/DJY1 exhibited typical semi-sterility, and the type of pollen abortion exhibited stained abortive. At the same time, we examined the DJY1 and F1 pollens using scanning electron microscopy and transmission electron microscopy and found the stained abortive pollens in F1 hybrid have small volume and most of them are shrunken, which have no accumulations of starch granules. According to pollen fertility, we constructed a linkage map covering the S37 region by 743 plants randomly selected from F2 progenies. The results indicated that the S37 locus was located to 2.2 cM in the interval between NJ5 and G4. Finally, S37 was mapped to the region between HP14 and G21 using a large F2 population contained 18014 plants, with physical size of about 73 kb. K