丛枝菌根真菌对枳根净离子流及锌污染下枳苗矿质营养的影响.docVIP

 丛枝菌根真菌对枳根净离子流及锌污染下 枳苗矿质营养的影响# 肖家欣，杨慧* 5 10 15 20 25 30 35 40 （安徽师范大学生命科学学院） 摘要：盆栽实验研究了不同施 Zn 水平（0、300 和 600 mg/kg）下，接种丛枝菌根真菌 Glomus intraradices 对枳苗生长、Zn、Cu、P、K、Ca、Mg 分布的影响，并采用非损伤微测技术测 定分析了菌根化与非菌根化枳根净 Ca2+、H+、NO3-离子流动态。结果表明：（1）在不同 施 Zn 水平下，接种菌根真菌显著提高了枳苗地上部及根部鲜重；随着施 Zn 水平的提高， 菌根侵染率呈降低趋势，枳苗地上部与根部 Zn 含量呈增加趋势，且接种株根部 Zn 含量显 著高于未接种株（对照）。（2）接种株未施 Zn 处理的地上部 Cu、P、K、Mg 和根部 Cu 含量、施 600 mg/kg Zn 处理的根部 Cu 及施 300 mg/kg Zn 处理的根部 P 含量均显著高于对照， 而菌根真菌侵染对枳苗 Ca 含量并无显著性影响。（3）接种株未施 Zn 处理的根部距根尖端 0 μm 和 600 μm 处净 Ca2+流出速率、600 μm 处净 H+流入速率、2400 μm 处净 NO3- 流入速率均显著高于对照。 关键词：植物生理生态学；丛枝菌根真菌；枳；净离子流；锌；矿质营养 中图分类号：Q945 Effects of arbuscular mycorrhizal fungus on net ion fluxes in the roots of trifoliate orange(Poncirus trifoliata) and mineral nutrition in seedlings under zinc contamination XIAO Jiaxin, YANG Hui (College of Life Sciences, Anhui Normal University) Abstract: IA pot experiment was conducted to study the effects of arbuscular mycorrhizal (AM) fungus,Glomus intraradices, on growth, distribution of zinc(Zn), copper(Cu), phosphorus(P), potassium(K), calcium(Ca), and magnesium(Mg) in trifoliate orange(Poncirus trifoliata (L.) Raf.) seedlings under different Zn addition levels (0, 300 and 600 mg·kg-1). Meanwhile, a non-invasive micro-test technique was used to determine the net fluxes of Ca2+, H+ and NO3- along mycorrhizal and nonmycorrhizal roots of trifoliate orange seedings. The results show that, AM colonization significantly increased the fresh weight of shoots and roots under different zinc addition levels. With increasing Zn addition levels, the mycorrhizal colonization percentage decreased, while the Zn levels in the shoots and roots were enhanced progressively. Zn concentrations in the roots were significantly higher in AM seedlings than those in non-AM seedlings (control). Cu, P, K, and Mg concentrations in the shoots under no Zn addition, Cu concentrations in the roots under Zn addition level of 600 mg·kg-1 and P concentrations in the roots under Zn addition level of 300 mg·