电针对IBS内脏高敏感大鼠DRG神经元中P2X2、P2X3受体的调节作用.docVIP

 电针对 IBS 内脏高敏感大鼠 DRG 神经元中 P2X2、P2X3 受体的调节作用# 翁志军1，吴璐一1，吴焕淦1，郭欣欣1，谭琳蓥1，辛玉虎2，董明1，刘慧荣1** 5 10 15 20 25 30 35 40 （1. 上海市针灸经络研究所，200030； 2. 复旦大学附属肿瘤医院，200032） 摘要：观察 IBS 内脏高敏感大鼠脊髓 L6-S3 段 DRG 中 P2X2、P2X3 受体表达情况，探讨 P2X2、 P2X3 受体在内脏高敏感性中的作用；通过行为学 AWR 评分结合合募配穴（天枢、足三里 穴）电针对 P2X2、P2X3 受体表达的调节来探索合募配穴电针治疗 IBS 的可能作用机制。采 用结直肠球囊扩张法（CRD）制备大鼠 IBS 内脏高敏感模型；利用免疫组织化学方法检测 IBS 内脏高敏感大鼠脊髓 L6-S3 段 DRG 神经元中 P2X2、P2X3 受体在造模前后、合募配穴电 针治疗前后表达情况。造模后，模型组与正常组 AWR 评分相比，内脏敏感性升高，说明模 型制作成功；治疗后，电针组与模型组 AWR 评分相比，内脏敏感性降低，电针治疗 IBS 内 脏高敏感性有效；与模型组相比，电针组 P2X2、P2X3 受体表达降低，合募配穴电针治疗可 能通过降低 P2X2、P2X3 在 DRG 神经元中的表达来降低 IBS 大鼠的内脏高敏感性。 关键词：P2X2；P2X3；内脏高敏感；肠易激综合征；合募配穴；电针 中图分类号：R245.81 Effect of He-Mu combination electroacupuncture (ST25 and ST37) on expression of P2X2、P2X3 of the Dorsal Root Ganglion in IBS Chronic Visceral Hypersensitivity Rats WENG Zhijun1, Wu Luyi1, Wu Huangan1, Guo Xinxin1, Tan Linying1, Xin Yuhu2, Dong Ming1, Liu Huirong1 (1. Shanghai Institute of Acupuncture-Moxibustion and Meridians,Shanghai University of Traditional Chinese Medicine, ShangHai200030; 2. Fudan University Shanghai Cancer Center, ShangHai 200032) Abstract: To investigate the expression of P2X2、P2X3 of the spinal marrow L6-S3Dorsal Root Ganglion in IBS Chronic Visceral Hypersensitivity Rats, explore the function of P2X2、P2X3 in irritable bowel syndrome. combining abdominal withdraw reflection ratings with adjustment expression of P2X2、P2X3 of the Dorsal Root Ganglion after electroacupuncture at Both ST25 and ST37, which could seek the possible mechanisms of electroacupuncture treatment of irritable bowel syndrome.Irritable bowel syndrome rat model was made by internationally recognized method of colorectal distention stimulation of balloons[1].Immunohistochemistry was used to observe the expression of P2X2、P2X3 of the spinal marrow L6-S3 Dorsal Root Ganglion following rat model and electroacupuncture treatment.The threshold of visceral sense was significantly lower in model group than in normal group,and significantly higher in