多效唑暴露干扰雄性褐鲳鮋的精子发生.docVIP

 多效唑暴露干扰雄性褐鲳鮋的精子发生# 李进寿1,2，孙凌斌1，左正宏1，陈猛1，耿宏1，王重刚1** （1. 厦门大学海洋环境科学国家重点实验室，福建 厦门 361005； 5 10 2. 宁德师范学院生物工程系，福建 宁德 352100） 摘要：多效唑属三唑类杀菌剂，在农业上广泛地用做植物生长调节剂。但是，很少有研究涉 及到多效唑对鱼类的生殖毒性。本实验研究环境相关浓度的多效唑对雄性褐菖鮋性腺发育的 影响及其作用机制。在 10, 100 和 1000 ng/L 浓度的多效唑暴露 50 天后，尽管褐菖鮋的性 体比指数无显著性的变化，但精巢成熟精子数量减少，精原细胞与精母细胞数量的大量增加。 多效唑暴露导致γ-谷氨酰转肽酶(性腺支持细胞的标志物)的活性呈浓度依赖性降低，这可 能是对脑中的促卵泡激素（FSHβ）表达减少的结果。脑中多效唑的积累可能是影响精子发 生的主要原因，这个效应可能通过脑－垂体－性腺轴实现。 关键词：多效唑；性腺发育；褐菖鮋；机制 中图分类号：X 174 文献标识码:A 15 Exposure to paclobutrazol disrupts spermatogenesis in male Sebastiscus marmoratus Li Jinshou1,2, Sun Lingbin1, Zuo Zhenghong1, Chen Meng1, Geng Hong1, Wang Chonggang1 20 25 30 35 40 (1. State Key Laboratory of Marine Environmental Science,Xiamen University, FuJian XiaMen 361005 2. Department of Biological Engineering,Ningde Normal University, FuJian NingDe 352100) Abstract: Paclobutrazol (PBZ) is triazole-containing fungicides and widely used in agriculture as an efficient plant growth retardant. However, far fewer studies addressing the reproductive toxicity of PBZ on fishes are available. The present study was conducted to investigate the effects of PBZ at environmentally relevant concentrations on testicular development in male rockfish Sebastiscus marmoratus and to gain insight into its mechanism of action. After exposure to 10, 100 and 1000 ng/L PBZ for 50 d, although the gonadosomatic index did not show a significant alteration, there was a reduced number of mature sperm and an abundance of the late stages of spermatocysts in the testes. The activity of γ-glutamyl transpeptidase (which is used as a Sertoli cell marker) was decreased in a dose-dependent manner after PBZ exposure, which might be a result of the reduction in FSHβ expression in the brain. The toxicity induced by accumulation of PBZ in the brain might be primary cause impacting the spermatogenesis via brain–pituitary–gonad pathway. Keywords: Paclobutrazol; testicular development; Sebastiscus marmoratus; mechanism 0 引言 自从有机氯和有机磷杀菌剂被禁用以来，新型杀菌剂的