Efficient_intracellular_delivery_of_a_pro-apoptotic_peptide_with_a_pH-responsive_carrier.pdfVIP

Reactive Functional Polymers 71 (2011) 254–260 Contents lists available at ScienceDirect Reactive Functional Polymers journal homepage: /locate/react pH modulated layer-by-layer assembly as a new approach to tunable formulating of DNA within multilayer coating Xuefei Wang, Jiekan Sun, Jian Ji ⇑ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou 310027, China Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China a r t i c l e i n f o a b s t r a c t Article history: A rapid growth of the biodegradable multilayer of poly (L-lysine) (PLL) and DNA was constructed here by Available online 22 September 2010 alternating deposition of PLL at high pH and DNA at low pH. The exponential growth rate of the multi- layer was increased by increasing pH difference between the two deposition solutions. The pH modulated Keywords: layer-by-layer method provides an easy and rapid way not only to elevate DNA dose in multilayer coating Layer-by-layer but also to modulate the ratio of PLL to DNA within multilayer coating. Relatively higher transfection lev- Exponential growth els were achieved for plasmid degraded from the PLL/plasmid 9.5/5.0 (PLL and plasmid were alternatively DNA deposited at pH 9.5 and pH 5.0) and 10.0/4.6 (film was formed by alternative deposition of PLL at pH 10.0 N/P ratio Gene transfection and plasmid at pH 4.6) films when compared with that of PLL/plasmid