精子形态异常分析.pdfVIP

EvaluationofSpermAbnormality

KnowledgeReview

cattlepigssheephorses

Oblate

ellipse

Acrosome

Nucleus

Tail

Neck

Head“Tadpole”

Abnormalspermtype

Abnormalsperm:Allspermwithabnormalmorphologyand

structurearecalledabnormalsperm.

Headdeformities:large,thin,defective,pointed,headless,

double-headed,etc.

Neckdeformity:bulky,broken,doubleneck,etc.

Taildeformity:curl,gyration,rupture,doubletail,missing

tail,etc.

Protoplasmdroplet

DetectionMethod

Preparation

Apparatus:slide,dropper,tweezers,dyetank,counter,mirrorcleaningpaper,

beaker,bottlewashing,etc.

Reagents:semen,blueink(orredinkor0.5%gentianvioletsolution),96%alcohol,

etc.

Semen:Freshsemen;frozensemen.

DetectionMethod

1.Smear.Adropofsemenisdroppedononeendofaglassslidewitha

pipettinggun.Thetopoftheotherglassslideiscontactedtothesemendropata

35°angle.Thesemendropisdistributedinastripbetweenthecontactedgesof

thetwoglassslides.Thenmovethetopglassslidefromrighttoleftandspread

thesemenevenlyontheglassslidebelowit.

2.Drying.Thesmeardriesnaturallyintheair.

3.Fixation.Fixedin96%alcoholfor5to6minutes.Takingitoutandrinsing,

thendryitintheshade.

4.Staining.Dyewithblue(red)inkfor3to5minutes,rinsingwithslow

streamwaterthendryit.

5.Microscopicexamination.Thesmearisobservedundera