IHC与FISH在乳腺癌HER-2蛋白表达和基因扩增检测中的应用.docxVIP

IHC与FISH在乳腺癌HER-2蛋白表达和基因扩增检测中的应用

摘要：目的：分析免疫组织化学法（IHC）与荧光原位杂交技术（FISH）在乳腺癌人表皮生长因子受体-2（HER-2）蛋白表达和基因扩增检测中的差异性分析。方法：回顾性选取2019年至2022年本院的158例乳腺癌患者作为研究对象，观察荧光原位杂交及免疫组织化学检测HER-2有无表达差异。结果：免疫组化化学检测有5例阴性（1+），

原位杂交结果显示没有扩增，阴性符合率100%。IHC检测有97例（2+），FISH检测结果有17例扩增，符合率17.52%。IHC检测有56例阳性（3+），FISH检测结果显示全部扩增，阳性符合率100%。提示HER-2通过免疫组织化学及荧光原位杂交技术检测，其表达有较好的一致性，且经过Kappa分析，k=0.775，一致性好（P0.05）。结论：荧光原位杂交与免疫组织化学方法在乳腺癌患者HER-2表达检测中，FISH稳定性更好，而IHC易受主观性、技术性等因素的干扰，必要时需结合两种方法检测。

关键词：乳腺癌；人表皮生长因子受体2；荧光原位杂交法；免疫组化法

DifferencebetweenIHCandFISHindetectingHER-2proteinexpressionandgeneamplificationinbreastcancer

Abstract:Objective:Toinvestigatethedifferencebetweenimmunohistochemistry(IHC)andfluorescenceinsituhybridization(FISH)indetectinghumanepidermalgrowthfactorreceptor-2(HER2)proteinexpressionandgeneamplificationinbreastcancer．Methods:HER2proteinexpressionandgeneamplificationin158breastcancerpatientsinAffiliatedZhongshanHospitalofDalianUniversityweredetectedbyIHCmethodandFISHmethodrespectivelyFromFebruary2019toJanuary2022.Results:Amongthe158breastcancerpatients,in56caseswithpositiveIHC(3+),56caseshadpositiveFISHtest，withthepositivecoincidencerateof100%．Amongthe97caseswithIHCequivocal(2+)17werepositiveforFISHtest,withthepositivecoincidencerateof17.52%.TheresultsshowedthatinadditiontoIHC(2+),theHER2proteinexpressiondetectedbyIHCwasconsistentwiththeHER2geneamplificationdetectedbyFISH．Amongthe5werenegativeforIHC(1+)and5werenegativeforFISHtest，withthenegativecoincidencerateof100%.Thereasonsforthedifferencebetweenthetwodetectionmethodsincludedsampleswasnotfixedintime，lowconcentrationofprimaryantibodyandupdatedguidelines．Conclusion:TheconsistencybetweenIHCandFISHmethodwasbetter．Inclinicalpractice，genetictestingmethodscanbeselectedaccordingtotheactualsituation,toguidingclinicaltreatme