姜黄的药理作用.pdf

Hepaticprotectionand

anticanceractivityof

curcuma

Content

•Introduction

•Materialsandmethods

•Result

Introduction

TurmericisCurcuma

Genusturmericplant

(CurcumalongaL.)dry

roots.Modern

pharmacologicalstudies

haveshownthatturmeric

hashepaticprotectionand

anticanceractivity.

Turmerone,amajorsesquiterpenoidincurcumaoil,

hasdemonstratedbothanti-inflmmationand

anticancereffects.Turmeronecannotonlysuppress

cyclooxygenase(COX-2)andnitricoxidesynthase

(iNOS),butalsoinduceapoptosisinvariouscancer

celllines.Inaddition,turmeroneshowed

immunomodulatoryactivitieswhichcouldcontribute

totheanticancereffect.

Materialsandmethods

Constructionofthetwomodels:

ConA-inducedhepaticinjurymodel

Orthotopicmouselivercancermodel

ConA-inducedhepaticinjurymodel

MaleC57miceweredividedinto3groups:

ConA+curcumaoilgroup:pretreatedwithcurcuma

oilat100mg/kg(i.p.)for3daysfollowedbya

treatmentofConAat30mg/kg(i.v.,tailvein)

ConAgroup:pretreatedwithsaline(samevolumeas

curcumaoil,i.p.)for3daysandthentreatedwithCon

Aat30mg/kg(i.v.,tailvein)

Thecontrolgroup:treatedwithsalineonly

Orthotopicmouselivercancermodel

Eight-week-oldC57L/Jmicewerehousedfiepercage,given

standardcommercialchow(20)andtapwaterandmaintained

ona12-hlight/darkcycle.Hepa1-6cellsweremaintainedat

37˚C,95%airand5%CO2inDulbeccosmodifidEagles

medium(DMEM),supplementedwith10%FBS.TheHepa1-

6cellswereinjectedintotherightflnkofC57L/Jmiceat

1x107in100µl.Whenthetumorswerereache