3组常见马源性成分鉴定引物特异性和灵敏度比较.pdfVIP

第14卷第5期 食品安全质量检测学报 Vol. 14 No.5 2023年3月 JournalofFood Safet yandQuality Mar.2023 3组常见马源性成分鉴定引物特异性和 灵敏度比较 夏慧丽」，马新宇？，潘映秋，黄雅芳² ？，管峰2* 310018) 318000；2.中国计量大学生命科学学院，杭州 （1.台州市食品检验检测中心，台州 摘要：目的 比较筛选基于聚合酶链式反应(polymerase chain reaction，PCR)技术的马源性成分鉴定方法中 去选取来自国标法、专利和文献报道的3组马源性成分鉴定引物，比较其灵敏 高灵敏度和特异性引物。方法 度和特异性，筛选高灵敏度和特异性引物。结果3组引物的灵敏度和特异性存在差异，以线粒体DNA为靶 标的I组马源性成分鉴定引物的特异性和灵敏度优于分别以线粒体和核基因组DNA为靶标的马驴通用鉴定引 物，检出最低限度均满足检测需求。结论通过比较马源性成分鉴定的3组PCR引物，以线粒体DNA为靶 标的I组马源性成分特异性鉴定引物可作为马成分鉴定的首选，线粒体和核基因组靶标联合可提高马成分鉴 定的准确性并可用于杂交种鉴定，为马源性成分及产制品鉴定提供了一定基础。 关键词：马源性成分；真伪鉴定；引物；特异性；灵敏度 Comparison of specificity and sensitivity of common primers for identification of horse derived components in 3 groups 2* XIA Hui-Lil, MA Xin-Yu², PAN Ying-Qiu, HUANG Ya-Fang’, GUAN Feng (1. Taizhou Food Inspection and Testing Center, Taizhou 318000, China; 2. College of Life Sciences, China Jiliang University, Hangzhou 310018, China) ABSTRACT: Objective To compare and screen the highly sensitive and specific primers in polymerase chain reaction (PCR) based methods for identification of horse derived components. Methods Three groups of primers for identification of horse derived components from Chinese national standard method, invention patent and literature report were selected, and the sensitivity and specificity were further compared to screen highly sensitive and specific