亚硒酸钠硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外（职称论文资料）.docVIP

亚硒酸钠硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：亚硒酸钠硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外 1 1 材料与方法 4 2 结果 6 3 讨论 7 文2：连翘提取物对小鼠腹腔巨噬细胞体外吞噬和NO释放的影响 8 1 材料和方法 9 2 结果 11 3 讨论 13 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 亚硒酸钠硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外（职称论文资料） 文1：亚硒酸钠硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外 A Study about the Effect of Sodium Selenite and Magnesium Sulfate on Cytokine Releasing and the Hydroxyproline Synthetizing Induced by Quartz CHEN Guo-yin，TAN Wei-jun*，YIN Hai-rong，HE Zheng，SUN Yan（School of Public Health，Southeast Univeity，Nanjing 210009，China） Abstract：［Objective］ To investigate the effect of sodium selenite and magnesium sulfate which were used separately on TNF-α and IL-1 releasing from macrophage treated with quartz and which were used separately and/or combined on the hydroxyproline synthetizing in human embryonic lung fibroblast also treated with quartz. ［Methods］ and human embryonic lung fibroblast were cultured at 37℃ with 5% carbon dioxide. were prepared during the growth period of logarithm in the concentration of 2×105 /ml and poisoned with quartz（200 mg/L），at the same time sodium selenite and magnesium sulfate in different concentration were added separately. After 24 hou，TNF-α and IL-1 in culture fluid were measured .Then the culture fluid and the human embryonic lung fibroblast were cultured together. After 48 hou，the hydroxyproline in human embryonic lung fibroblast culture fluid were measured. In addition，when the was poisoned with quartz，sodium selenite and magnesium sulfate were added together simultaneously. Then followed the same procedure as above mentioned and the hydroxyproline in human embryonic lung fibroblast culture fluid were measured too after 48 hou. ［Results］ ①Magnesium sulfate could reduce TNF-α releasing，with the middle dose（ μmol/L）most effective，but sodium selenite could not.②Sodium selenite and magnesium sulfate both could reduce IL-1 releasing，with the low dose（ μmol/L）and