反应工程基础：Chap7 Reaction Mechanisms, Pathways,.pptVIP

7.4.5 Wash-out Flow rate at which wash-out occurs Maximum rate of cell production Cell concentration and production rate as a function of dilution rate DCC Dmaxprod D CS CC 7.4.8 Oxygen-limited growth Oxygen transfer rate (OTR) 7.4.9 Scale-up 其它内容不讲。 Summary In the PSSH, the rate of formation for the active intermediates equals zero. If the active intermediate A* is involved in m different reactions, Active intermediate: highly reactive and present in low concentrations 2. The azomethane (AZO) decomposition mechanism is 3. Enzyme kinetics: PSSH for (E?S) Michaelis-Menten equation 4. Three different types of inhibition – competitive, uncompetitive, and mixed inhibition Noncompetitive (both slope and intercept change) Uncompetitive (intercept changes) Competitive (slope changes) No inhibition 1/(-rs) 1/S 5. Bioreactors: Cells + Substrate ? More cells + Product (a) Phases of bacteria growth: I. Lag; II. Exponential; III. Stationary; IV. Death (b) Monod growth rate law (c) Stoichiometry (d) Unsteady-state mass balance on a chemostat Product-enzyme complex PSSH to both (E?S) and (E?P) : Briggs-Haldane equation 7.2.4 Batch reactor calculation for enzyme reactions Mole balance (Liquid phase) Rate law Combine Integrate Michaelis-Menten equation in terms of the substrate concentration Intercept = Vmax/KM (S0-S)/t [ln(S0/S)]/t 0 Slope = -1/KM Evaluating Vmax and KM Effect of temperature T Vmax 7.3 Inhibition of enzyme reactions Factorstemperaturesolution pHinhibitor Inhibitorspecies that interact with enzymes and render the enzyme ineffective to catalyze its specific reaction Reversible inhibitions:competitive, uncompetitive, noncompetitive （竞争） （无竞争） （非竞争） 7.3.1 Competitive inhibition Competitive inhibition pathway I + E?I KI Reaction steps (1) (2) (3) (4) (5) PSSH Lineweaver-Burk plot for competitive inhibition No inhibition 1/(-rs) 1/S Competitive inhibition Increasing inhibitor concentration (I) 7.3.2 Uncompetitive inhibition I + E?S?I KI (1) (2)