液质联用的定性与定量(很好自己合并的PDF).docx

AS噩編 串联液质系统分析原理 美国应用生物系统中国公司 应用技术服务部 Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC Date I AB CONFIDENTIAL ? 2006 Applied Biosystems Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC Date I AB CONFIDENTIAL ? 2006 Applied Biosystems What is a mass spectrometer ? 质谱技术： ?质谱分析过程：样品通过进样系统进入离子源，由于结构 性质不同而电离为各种不同质荷比(m/z)的分子离子和碎 片离子，而后，带有样品信息的离子碎片被加速进入质量 分析器，不同的离子在质量分析器中被分离并按质荷比大 小依次抵达检测器，经纪录即得到按不同质荷比排列的离 子质量谱，也就是质谱(mass spectrum)o ?质谱：按离子的质荷比的大小依次排列形成的图谱 AOYANCINGSCICNCC AOYANCING SCICNCC Date I AB CONFIDENTIAL ? 2006 Applied Biosystems AOYANCINGSCICNCC AOYANCING SCICNCC Date I AB CONFIDENTIAL ? 2006 Applied Biosystems 直接进样GC 直接进样 GC LC CE MALDI 「ESI APl{ APCI LAPPI 四极杆：Quadrupole 离子阱、线性离子阱：IT, LIT 飞行时间：TOF 傅立叶变换离子回旋共振：FT-ICR Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC 8 Date | AB CONFIDENTIAL ? 2006 Applied Biosystems Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC 8 Date | AB CONFIDENTIAL ? 2006 Applied Biosystems 高效分离系统 高灵敏度检测系统 GC 大量的载气 曰、Cl… MS 高真空体系 HPLC 大量的液态流动相 ? MS/MS 高真空体系  液相色谱与质谱的联用技术 ?主要障碍: 1、 能否把大量的溶剂除去并不使质谱仪受到污染 GS21、2、GS1上面两点是影响灵敏度的主要因素IGS2TEM2、 能否使被分析样品在温和条件下 GS2 1、 2、 GS1 上面两点是影响灵敏度的主要因素IGS2 TEM GS2 f , TEM J H2O比例大时，TEM f ,有利于离子化 Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC 8 Date | AB CONFIDENTIAL ? 2006 Applied Biosystems Opportunities in the Services and System Solutions Market Opportunities in the Services and System Solutions Market /(M曜ms AOYANCINGSCICNCC AOYANCING SCICNCC 8 Date | AB CONFIDENTIA