APN-AMPK信号通路调节骨代谢作用于激素性股骨头坏死的机制研究.docxVIP

APN-AMPK信号通路调节骨代谢作用于激素性股骨头坏死的机制研究 张翔　吴泱　董晓俊　张鸿振　王萧枫　梅凌　张涛 [摘要] 目的 通過观察激素性骨坏死模型造模前后骨代谢变化，阐述干预APN-AMPK信号通路调节骨坏死的作用机制。 方法 共20只健康成年白兔，随机分为正常组和造模组，每组10只，应用LPS联合醋酸泼尼松龙诱导激素性股骨头坏死模型，疗程为9周。实验过程中记录动物一般情况，造模前后分别对所有兔耳缘静脉取血2 mL，进行ELISA检测;通过病理组织学检查确定造模成功，同时分别进行骨细胞AMPK mRNA表达、TNF-α/OPG相关性分析。 结果 造模后，造模组兔血浆APN水平（2.49±0.48）ng/mL，较同组造模前（6.74±0.04）ng/mL比较明显降低，差异有统计学意义（P0.05）;造模组兔骨细胞AMPK mRNA表达为（0.364±0.017），较造模前显著降低，差异有统计学意义（P0.05）。此外，血清APN含量和AMPK mRNA表达与OPG含量呈正相关性，与TNF-α表达呈负相关性。 结论 脂联素体内含量可直接引起骨坏死疾病进程，也许APN-AMPK信号通路在疾病转归过程中承担重要角色，通过干预APN-AMPK信号通路正向传导，可以为临床治疗激素性股骨头坏死提供新的思路和方法。 [关键词] 激素性股骨头坏死;APN-AMPK信号通路;动物模型;机制研究 [中图分类号] R580 [文献标识码] A [文章编号] 1673-9701（2020）21-0035-05 Study on mechanism of APN-AMPK signal pathway regulating bone metabolism in steroid-induced femoral head necrosis ZHANG Xiang1 WU Yang2 DONG Xiaojun3 ZHANG Hongzhen1 WANG Xiaofeng1 MEI Ling3 ZHANG Tao4 1.Department of TCM Orthopedics，Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine in Zhejiang Province， Wenzhou 325000， China; 2.Internal Medicine of TCM，Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine in Zhejiang Province， Wenzhou 325000， China; 3.Department of Orthopedics， Wuhan Hospital of Traditional Chinese Medicine， Wuhan 430014， China; 4. Hubei University of Traditional Chinese Medicine， Wuhan 430014， China [Abstract] Objective To expound the mechanism of regulating osteonecrosis by intervening APN-AMPK signal pathway through observing the changes of bone metabolism before and after the modeling of steroid-induced femoral head necrosis. Methods A total of 20 healthy adult rabbits were randomly divided into the normal group and the model group，with 10 rabbits in each group. LPS and prednisolone acetate were used to induce the steroid-induced femoral head necrosis. The course lasted for 9 weeks. During the experiment，the general conditions of the animals were recorded. Two ml of blood was taken from the ear veins