心血管实验病理学方法简介.ppt

3.7 肺动脉高压的组织学测量 腺泡前肺动脉 /肌性动脉（终末细支气管以上） 腺泡内肺动脉 /肌性或半肌性动脉（终末细支气管以下），在肺血流动力学中作用更重要。 肌型、半肌型和无肌型动脉的构成（%） 管径100 μm动脉中，肌型动脉所占百分比 3.8 心血管植入器材的病理学评价 主要试验器材 人工瓣膜 血管内支架 人工血管 瓣膜成形环 封堵器 心脏(起搏)电极 射频或冷冻治疗仪 3.8 心血管植入器材的病理学评价 评价原则 生物相容性为主、疗效评价为辅 规范剖解，注意大体改变 扫描电镜为必选项目 注意区分手术损伤、异物反应、自发疾病 注意事项 熟悉器材结构和术式 保持心腔和血管腔清洁（抗凝/涮洗） 禁止用力牵拉和挤压血管 可降解支架的降解过程（偏振光形态观察） 成型环的临床前病理学评价 主动脉支架与夹层封堵器 室间隔封堵器的临床前病理学评价 * Concentration of unknown samples are determined from a standard curve STD concentration values are obtained when the interaction between * Saturation radioligand binding experiments measure specific radioligand binding at equilibrium at various concentrations of the radioligand. These experiments are performed to determine receptor number and affinity on cells but also between radiolabeled antigen and Ab. This can take anywhere from a few minutes to many hours, depending on the ligand, receptor, To, and other experimental conditions. The lowest concentration of radioligand will take the longest to equilibrate. When testing equilibration time, therefore, use a low concentration of radioligand (perhaps 10-20% of the KD). Nonspecific binding is almost always a linear function of ligand concentration. * Trypan Blue/ Cardiovasc Res. 2009 Nov 1;84(2):273-82 * * 灌注区蓝色 缺血区不着色 梗死大小= 梗死面积/全心面积 梗死面积/左室面积 梗死面积/缺血危险区面积 * 组织切片法 Circulation. 2010 Sep 14;122(11 Suppl):S107-17 * Histology of the constrictor: a 3 weeks after implantation of the ameroid constrictor the coronary artery is necrotic with purulent inflammation and granulation tissue is spreading from the periphery (H.-E. stain). b 3 weeks after implantation of the ameroid constrictor the distal part of the coronary artery shows segmental inflammatory and degenerative lesions: transmural vacuolization and destruction, severe intimal hyperplasia and mild purulent inflammation (Picro Sirius Red stain). * 2.3.4 特殊的电镜技术免疫金与纳米金标记比较 2.3.4 特殊的电镜技术/免疫金标记 Dystrophin 2.3.4 特殊的电镜技术免疫金标记（双标，10 nm，20 nm） 2.3.4 特殊的电镜技术/纳米金标记 纳米金颗粒与免疫球蛋白Fab’片段结合而成