流式在干细胞中的应用 武汉用户会议.pptx

流式卓越性能：助推干细胞研究新发现Stem cell definitionstem cell researchipsembryonic stem cellcancer stem cellsomatic stem cellHSCheart MSCmusclemammarybraincolon/intestinalskin liver lung ……(inducible pluripotent stem cell)leukemiaosreosarcomaMMbreastmelanomacolon/intestinal cancerliver lung ……How to do stem cell researchwhat(identification and purification)three questions:howwhere(mechanism and regulation )(niche homing and expansion)Future aim:therapy(cell transplantation and gene modification )Flow Cytometry in Stem Cell BiologyFlow cytometry and cell sorting are indispensable techniques for both the identification and isolation of embryonic and adult stem cells in both human and non-human bone marrow and other tissues. Cell surfacemarkersPhysiologicalmarkersIntracellularproteinsFluorescent immunophenotyping for stem cell markersAldehyde dehydrogenase activity detection using fluorogenic substratesPump substrate efflux, including Hoechst side population analysisMainly transcription factors that can be immunolabeledFlow cytometry and cell sorting is a powerful and useful tool for research applications流式在干细胞功能和机制研究中表现卓越流式技术在造血干细胞功能和机制研究中的应用流式技术在肿瘤干细胞功能和机制研究的应用流式技术在ips研究中的应用流式技术在其他干细胞研究领域的应用流式在干细胞功能和机制研究中表现卓越流式技术在造血干细胞功能和机制研究中的应用流式技术在肿瘤干细胞功能和机制研究的应用流式技术在ips研究中的应用流式技术在其他干细胞研究领域的应用what(identification and purification)howwhere(mechanism and regulation )(niche homing and expansion)therapy(cell transplantation and gene modification )what is stem cell ?干细胞鉴定金标准：只要一个细胞就能再生整个系统流式分选单个细胞 活性高what(identification and purification)howwhere(mechanism and regulation )(niche homing and expansion)therapy(cell transplantation and gene modification )what is hematopoietic stem cell ?HematopoesisThe “standard” Weissman model The CLP/CMP dichotomy might be more flexible.Self-renewingNon self-renewingStem cell phenotypic analysis Mouse Flk2 = Flt-3 = CD135 Slamf1 = CD150 FcγR = CD16/CD32 HumanLin- CD34- CD38- CD90low CD45-LT-HSCLin- c-kit+ Sca-1+ Flt3- CD34-Thy1.1lo CD150+ CD48- CD244-HSCsST-HSCLin- CD34