蛛网膜下腔出血后S100B蛋白表达增加与脑血管-第三军医大学学报.doc

蛛网膜下腔出血后S100B蛋白表达增加与脑血管痉挛有关 陈果作者简介：陈果（1973-），男，重庆人，硕士研究生，主治医师，主要从事脑血管疾病的外科治疗研究，电话0232075作者单位：400010 重庆市，重庆医科学大学附属第二医院神经外科通讯作者：马颖 E-mail： maying1964@ , 马颖,程远 作者简介：陈果（1973-），男，重庆人，硕士研究生，主治医师，主要从事脑血管疾病的外科治疗研究，电话0232075 作者单位：400010 重庆市，重庆医科学大学附属第二医院神经外科 通讯作者：马颖 E-mail： maying1964@ （重庆医科大学第二临床学院神经外科 重庆400010） 提 要： 目 的：通过对蛛网膜下腔出血（subarachnoid hemorrhage，SAH）后不同时间点S100B蛋白在基底动脉中表达水平的研究，探讨S100B蛋白与CVS关系，进一步阐述SAH后CVS的发生机理。方 法： SD大鼠40只，分为正常对照组、假手术组、生理盐水组和实验组。根据取材时间不同，又将实验组分为SAH后第1d、3d、4d、7d、14d组，病理切片观察基底动脉病理改变；测定S100B蛋白染色含量，并比较不同时间点基底动脉中S100B蛋白的表达；进行统计学分析。结果：SAH后在第3天时出现明显的血管腔变小；7天时血管痉挛达高峰；14天时动脉改变较7天时有减轻；基底动脉中S100B蛋白主要表达位于平滑肌细胞。SAH后第3d开始表达增强，7d时表达最强，14d时减弱。结 论：SAH后不同时间点S100B蛋白的表达强度不一，随着CVS加重表达越强，在第7d达高峰。因此S100B蛋白的表达可能与CVS有关。 关键词：SAH，CVS，S100B蛋白，免疫组化染色 Relationship between S100B protein expression and cerebral vasospasm after subarachnoid hemorrhage in rats CHEN Guo, MA Ying, CHENG Yuan, CHEN Wei-fu, XIE Zong-yi, ZHU Feng-jun (Department of Neurosurgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China) Abstract: Objective To explore the mechanism of cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) by investigating the relationship between S100B protein and CVS. Methods Forty healthy SD rats were divided into four groups randomly: SAH groups, sham operation group, saline group and normal control group. On day 1, 3, 4, 7, 14 after SAH establishment, the basal blood vessels were observed under light microscope and S100B protein expressions in basal arteries were analyzed by immunohistochemical staining. Results CVS happened on day 3 after SAH, and reached the peak on day 7 and relieved on day 14. S100B protein mainly expressed in smooth muscle of basal arteries, whose expressions became stronger on day 3 after SAH and reached the climax on day 7 and got declined on day 14. Conclusion S100B protein expressed in different levels during the occurrence of SAH. As CVS got aggravated, the