六角果鸢尾离体快繁技术体系的构建-热带亚热带植物学报.PDFVIP

热带亚热带植物学报 2018, 26(4): 415 ~ 420 Journal of Tropical and Subtropical Botany 六角果鸢尾离体快繁技术体系的构建 戴长志, 何平, 张映雪, 尹增芳* (南京林业大学南方现代林业协同创新中心；生物与环境学院，南京 210037) 摘要：为构建六角果鸢尾(Iris hexagona) 的离体快繁体系，以其幼嫩根状茎为外植体，研究了培养基和植物生长调节剂对不 定芽诱导、增殖和植株生根的影响。结果表明，根状茎用 0.1% HgCl2 消毒 13 min 的效果较佳；不定芽诱导最适培养基为 MS+ 6-BA 1.5 mg L– 1 +NAA 0.5 mg L– 1+ 蔗糖30 g L– 1 + 琼脂7.5 g L– 1，不定芽增殖的适宜培养基为MS+ 6-BA 0.5 mg L– 1 +NAA 0.2 mg L– 1 + KT 0.3 mg L– 1+ 蔗糖30 g L– 1+ 琼脂7.5 g L– 1；在MS+IBA 1.5 mg L– 1 + 蔗糖30 g L– 1 + 琼脂7.5 g L– 1 培养基上不定 芽生根率可达 100%；腐殖土和珍珠岩+泥炭土+ 蛭石(1 ∶2 ∶1)均可作为组培苗移栽的适宜基质，移栽成活率可达100%。 关键词：六角果鸢尾；离体快繁；培养基；植物生长调节剂 doi: 10.11926/jtsb.3847 Construction of Rapid Propagation System in vitro of Iris hexagona * DAI Chang-zhi, HE Ping, ZHANG Yin-xue, YIN Zeng-fang (South Forestry Modern Forestry Collaborative Innovation Center, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China) Abstract: In order to construct the rapid propagation system in vitro of Iris hexagona , the effects of medium and plant growth regulators on induction, proliferation and rooting of adventitious buds were studied by using young rhizome as explant. The results showed that the rhizome was sterilized for 13 min with 0.1% HgCl . The optimum 2 – 1 – 1 – 1 – 1 medium for induction was MS+1 .5 mg L 6-BA+ 0.5 mg L NAA+ 30 g L sucrose+ 7.5 g L agar, and that – 1 – 1 – 1 – 1 for adventitious bud proliferation was MS+0.5 mg L 6-BA+ 0.2 mg L NAA+ 0.3 mg L KT+ 30 g L –