6株人源乳酸菌体外抗氧化活性比较.PDFVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.7 6 株人源乳酸菌体外抗氧化活性的比较 黄玉军，刘冬，赵兰凤，顾瑞霞 （江苏省乳品生物技术与安全控制重点实验室，扬州大学食品科学与工程学院，江苏扬州 225127） 摘要：本文对分离自江苏如皋长寿村人群肠道的6 株乳酸菌体外抗氧化活性进行了研究。测定了6 株菌对过氧化氢耐受能力、 不同组分对羟自由基、DPPH 自由基清除能力、还原能力、抗脂质过氧化能力及T-SOD 和GSH-Px 活性，结果表明：6 株菌中，发酵 乳杆菌L2 、L4 ，屎肠球菌E2 对1.0 mM H O 耐受能力较强。6 株菌不同组分均具有一定的抗氧化活性，发酵上清液的抗氧化活性总 2 2 体要优于菌体和胞内提取物。其中，L2 发酵上清液羟自由基清除能力和T-SOD 活性最强；E2 发酵上清液DPPH 自由基清除能力和 GSH-Px 活性最高；发酵乳杆菌L1 发酵上清液还原能力最高；干酪乳杆菌L3 发酵上清液抗脂质过氧化能力最强。测定结果表明这6 株菌总体抗氧化能力较好，具有潜在的应用价值。 关键词：乳酸菌；发酵上清液；自由基；氧化应激；抗氧化剂 文章篇号：1673-9078(2013)7-1518-1522 In Vitro Antioxidant Activities of Six Lactic Acid Bacteria Isolated from Human Intestinal Tract HUANG Yu-jun, LIU Dong, ZHAO Lan-feng, GU Rui-xia (Jiangsu Province Key Lab of Dairy Biotechnology and Safety Control College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China) Abstract: The antioxidant activities of 6 lactic acid bacteria isolated from the intestinal tract of the longevity village people of Jiangsu Rugao were studied by hydrogen peroxide tolerance assay, hydroxyl radical and DPPH radical scavenging assays, reducing activity assay, anti-lipid peroxidation assay, T-SOD and GSH-Px activity assays in this study. The results showed that L. fermentum L2, L. fermentum L4 and E. faecium E2 had higher resistant capability against 1.0 mM hydrogen peroxide than the others. Different constituents of 6 lactic acid bacteria had different antioxidant activities, among which the antioxidant activity of fermentation supernatant was superior to bacterial cell and intracellular extract. The fermentation supernatants of L. fermentum L2 showed the highest hyd