树舌发酵浸膏粗多糖对SW1116细胞生长抑制作用.PDFVIP

吉林农业大学学 2011，09 Journal of Jilin Agricultural University E-mail:jlndxb@ 树舌发酵浸膏粗多糖对SW1116 细胞的生长抑制作用∗ ∗∗ ** 刘小腊，余 涛 ，宋 慧 ，苏 玲，王 雪 吉林农业大学生命科学学院，长春 130118 摘 要：采用热水浸提树舌发酵浸膏中的水溶性多糖，苯酚-硫酸法测定多糖的含量，MTT 法测定GAFP对SW1116细 胞的生长抑制率，荧光染色观察细胞的核形变化，流式细胞仪检测细胞周期等方法，研究了树舌发酵浸膏粗多糖 （GAFP ）体外对人结肠腺癌细胞SW1116的生长抑制作用。结果表明：与正常Vero 细胞相比较，GAFP对SW1116细 胞的生长抑制具有时间和剂量依赖性；荧光显微镜下观察到经GAFP处理的细胞呈现出凋亡早、晚期的染色质变化； 流式细胞仪检测结果显示GAFP 能将SW1116细胞阻滞于细胞周期的G2 /M期。结论：GAFP通过影响DNA 的合成和阻 滞细胞周期达到体外抑制SW1116细胞生长的作用。 关键词：GAFP ；苯酚-硫酸法；体外抑瘤；MTT 法；细胞周期 中图分类号：Q71 文献标识码：A 文章编号：1000-5684(2011) DOI: CNKI:22-1100/S1104.002 /kcms/detail/22.1100.S1104.002.html 网络出版地址： Inhibitory Effect of Crude Polysaccharides on Growth of SW1116 Cells from Submerged Fermentation of Ganoderma applanatum (Pers.) Pat LIU Xiao-la，YU Tao，SONG Hui，SU Lin，WANG Xue College of Life-Science, Jilin Agricultural university, Changchun 130118, China Abstract: To explore inhibitory effect of GAFP on growth of human adenocarcinoma of colon (SW1116) cells in vitro. GAFP was obtained through water extraction and the concentration of polysaccharides was determined with Phenol-Sulfuric acid method; using MTT to assay the inhibition ratio on growth of SW1116 cells; the changes of nucleus were measured with fluorescence staining and flow cytometry was used to investigate cell cycle. Compared to Vero normal cells, GAFP could obviously inhibit the proliferation of SW1116 with time and dose-dependent relation; besides, chromatin changes of early and late apoptosis in nucleus with fluorescence microscopy could be observed; FACS show that GAFP could induce G2/M phase arrest in cell cycle. GAFP inhibit the growth of SW1116, by the way of affecting DNA synthesis, arresting mitotic cycle on G2/M phase. Key words: