水稻稻瘟病拮抗菌L1鉴定与抑菌特性初步研究.PDFVIP

微生物学通报 JUN 20, 2008, 35(6): 898~902 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 水稻稻瘟病拮抗菌 L1 鉴定及抑菌特性的初步研究 1* 2 1 1 1 李永刚 宋兴舜 马凤鸣 文景芝 赵雪莹 (1. 150030) (2. 150040) 摘 要: 本研究对水稻稻瘟病菌的拮抗菌L1 形态、生理生化特性等进行测定, 结果发现其为杆状 细胞、革兰氏阳性、菌落不规则有褶皱、好氧生长、颜色较深等, 初步鉴定为枯草芽孢杆菌; 拮 抗菌L1 培养5 d 的发酵液抑菌活性最高, 抑菌率为74.57%; 拮抗菌L1 对水稻稻瘟病菌的拮抗活 性物质对温度不敏感; 拮抗菌 L1 的发酵液用硫酸铵梯度沉淀法提取粗蛋白, 在 50%~60%硫酸铵 饱和度下沉淀的粗蛋白质对水稻稻瘟病菌抑菌效果最好, 平均抑菌半径达0.51 cm。 关键词: 生物防治, 枯草芽孢杆菌, 拮抗蛋白, 鉴定 Identification and Characterization of an Antagonistic Strain L1 Against Phyricularia grisea LI Yong-Gang1* SONG Xing-Shun2 MA Feng-Ming1 WEN Jing-Zhi1 ZHAO Xue-Ying1 (1. Agricultural College , Northeast Agricultural University, Harbin 150030) (2. Department of Genetics, College of Life Science, Northeast Forestry University, Harbin 150040) Abstract: On the basis of morphological, physiological and biochemical characterization, the antagonistic bacterium strain L1 against Phyricularia grisea was identified. The strain L1 was bacilliform, Gram-positive, fuscous and draped colonies, aerobiosis. The result showed that the strain L1 belongs to Bacillus subtilis. The antimicrobial activity of fermentation liquid reached the highest after 5 days-old cultures. And inhibitory rate accounted for 74.57%, but it had not changed along with the time increase. The activity effect of antim- icrobial substances was insensitive to heat. The filtrated Bacillus subtilis strain