低分子肝素分子量测定方法研究.pdfVIP

-size exclusion chromatography-mutiangle laser light scattering, SEC-MALLShigh performance size exclusion chromatography, HPSEC HPSEC TSKgel G2000SWXL 0.1 mol/L SEC-MALLS TSKgel G3000SWXL TSKgel G2000SWXL 0.1 mol/L 18 SEC-MALLS HPSEC HPSEC SEC-MALLS HPSEC HPSEC SEC-MALLS Comparative Study of Two Methods on Determination of Molecular Weight and Molecular Weight Distribution of Low Molecular Weight Heparin WANG Xin-yu, FAN Hui-hong(National Institutes for Food and Drug Control, Beijing 100050, China) Abstract: Objective: Comparative study on determination of molecular weight and molecular weight distribution of low molecular weight heparin by high performance size exclusion chromatography (HPSEC) and size exclusion chromatography in conjunction with mutiangle laser light scattering detector(SEC-MALLS). Methods: HPSEC, in conjunction with RI detector, was performed on a TSKgel G2000SWxl column, using 0.1 mol/L ammonium acetate as mobile phase. SEC-MALLS, in conjunction with both RI and MALLS detectors, was performed on a TSKgel G3000SWxl column in series of a TSKgel G2000SWxl column, using 0.1 mol/L ammonium acetate as mobile phase. Results: The weight average molecular weight calculated by 1 these two methods were almost the same. The number average molecular weight calculated by SEC-MALLS was higher than HPSEC, while the percentage of low molecular weight part was lower. Conclusion: The molecular weight calculated by HPSEC is accurate, but the calculation process is complex. SEC-MALLS can directly determine the absolute molecular weight of LMWH without molecular calibrants, which can be used to confirm the results obtained by HPSEC. Keywords: Low molecular weight heparin; HPSEC; SEC-MALLS; absolute molecular weight low mol