扩散皿吸收-盐酸滴定法测定牡丹籽中的蛋白质含量.pdfVIP

４７ ２ Vol．４７ No．２ ２０１８ ２ Applied Chemical Industry Feb．２０１８ - ， （ ， ５５００２５ ） ：-。 １０ mL 、０．２ g CuSO ４ ６．０ g K SO 。 ３ mL ２０ g／L ，５ mL ， ２ ４ １０ mL ４００ g／L NaOH ，，２４ h。 。 ， ２ １０ ～２００ μg／mL ，R ＝０．９９６ ６ ，７４．６２％ ～１０６．０７％， ０．２７％ ～２．８８％，４ １０％ ～１４％，，０．８４％。 、，，，。 ：；；； ：TQ ９３７ ；TQ ０１４ ；TQ ０１６．１ ：A ：１６７１ －３２０６（２０１８）０２ －０４０６ －０４ WU Fang ，SUN Rong-guo （College of Chemistry and Materials Science，Guizhou Normal University，Guiyang ５５００２５ ，China） ：A simple and reliable preparation method with conway diffusion absorption and muriatic acid ti- tration detection for mass determination protein in the peony seeds has been established．Under catalyst （０．２ g CuSO and ６．０ g K SO ） in electric hot plate with １０ mL concentrated sulfuric acid to heat，then ４ ２ ４ took ５ mL extracts and １０ mL ４００ g／L sodium hydroxide in diffusion cell，３ mL ２０ g／L boric acid absorp- tion in the inner chamber and then covered with cap．Afterwards keep it in the oven for ２４ h，finally，titra- tion with a known concentration muriatic acid．It’s results was about ０．８４％ significant difference compare with Kjeldah method．The linear calibration ranges of ammonium sulfate were within １０ ～２００ μg／mL，the ２ results showed good linearity （R ＝０．９９９ ６）．Average recoveries were ７４．６２％ ～１０６．０７％，the corre- sponding relative standard deviations were ０．２７％ ～２．８８％．It was demonstrated that the proposed method was good repeatability，stable and particularly suitable for d