短花针茅snp标记开发与遗传多样性研究草学专业论文.docxVIP

塑鍪壹奎兰堡主兰垡丝奎 塑鍪壹奎兰堡主兰垡丝奎 望垄盐量!!!堡里茎茎兰望堡垩竺垄 3．短花针茅具有中等偏低水平的遗传多样性。在物种水平上，期望杂合度 是O．1308，Nei氏遗传距离为O．1306，遗传变异主要发生在群体内部(91．49％)； 在群体水平上，期望杂合度为O．122；短花针茅基因流为2．504，表明群体间具有 较充分的基因交流。 4．短花针茅群体间遗传距离和地理距离具有显著正相关关系。8个短花针 茅群体可分为4组，组间的遗传变异所占比例相对较少。 本研究为进一步剖析短花针茅遗传多样性、遗传结构及种群动态变化奠定 了基础数据。 关键词：短花针茅；遗传多样性；遗传结构；SNP标记；转录组测序 万方数据 内蒙古大学硕士学位论文 内蒙古大学硕士学位论文 短花针茅SNP标记开发与遗传多研究 SNPS DEVELOPMENT AND GENETIC DIVERSITY OF^Sr删脚约咿乙()尺／4 POPULATION ABSTRACT Biodiversity is the base of survival and development of human beings，the research and protection ofbiodiversity has been one ofthe most hot topics in the world． Biodiversity is not only the source of adaption and evolution，but the essential nature resource．Therefore，the study of biodiversity plays an indispensible role in the theoretical knowledge and practical application．Our experiment samples came from 8 Stipa breviflora populations(30 individuals of each population)，which were collected from Inner Mongolian Plateau，Ordos Plateau，Alxa Plateau，Loess Plateau and Qinghai—Tibet Plateau．We sent 8 samples，coming from 8 different populations，to conduct transcriptome sequencing．By transcriptome sequencing，analyzing the results of sequencing，identifying SNP sites，designing the primers of SNP,utilizing the PCR to filter and amplify the primer,we developed SNPs．At last，we used these SNPs to illuminate the genetic diversity and genetic structure of the S．breviflora population and to explore the related influence factors．The main results of our study are as follows： 1．We succeeded to construct relatively completed transcriptome data set of S． breviflora．There were 60 1 1 1 6 1 1 2 raw reads in total，which mean length was 1 50bp from 8 samples．After filtering the low quality reads，we got 55 1 569834 clean reads． There were 8273 5475 1 00 high quality basic groups，taking the 9 1．76 percents of the total sequencing data，which contained the number of GC r