mtor信号通路在牛乳腺上皮细胞甘油三脂和脂肪酸合成中的作用微生物学专业论文.docxVIP

mTOR信号通路在牛乳腺上皮细胞甘油5一_-LI“J日,“T”IJ613肪酸合成中的作用 mTOR信号通路在牛乳腺上皮细胞甘油5一_-LI“J日,“T”IJ613肪酸合成中的作用 表达，甘油三脂合成增多(pO．01)；(5)5 mg／ml胰岛素刺激细胞12 h，甘油三 酯合成量与对照组相比，有显著性提高(pO．05)，rapamycin对这一过程有抑制 作用；(6)胰岛素诱导细胞内脂肪酸的合成；(7)短时间(2 h)无乳链球菌侵染 细胞诱导甘油三酯合成和分泌(pO．01)，使脂肪酸的组成发生变化。 综上所述，本实验证明mTOR信号通路在牛乳腺上皮细胞甘油三脂和脂肪酸 合成中发挥正向调控作用，其机制可能是调控了合成过程中关键酶的表达。胰 岛素诱导细胞内甘油三酯和脂肪酸的合成，而无乳链球菌侵染则导致细胞内脂 肪酸组成发生变化。这些过程中有mTOR信号通路参与，具体机制还需进一步研 究。 关键词：mTOR信号通路；甘油三酯；硼hil叱l合成；胰岛素；无乳链球菌 II 万方数据 内蒙古大学硕士学位论文Regulation 内蒙古大学硕士学位论文 Regulation of triacylglycerol and Fatty acids Synthesis by mTOR Signaling Pathway in BMECs Abstract The content and composition of milk fat IS closely related with nutritional quailty of cow’S milk．Triacylglycerol(TG)is the main component of cow’S milk fat． In mammary cells，the fatty acids utilized for TG synthesis is obtained from outside of the cell or synthesised de novo．The synthesis of milk fat in mammary cells is regulated by nutritional factors and various signaling pathways，in which mTOR signaling pathway plays an important role．Mastitis is the most common disease in dairy COWS，which affects the content of TG and the composition of fatty acid． S．agalactiae is a common pathogen of mastitis．In the present study,the synthesis and content of TG and fatty acids and regulatory role of mTOR signaling pathway were examed in bovine mammary epithelial cells in the condition of insulin stimulation and S．agalactiae infection． Bovine mammary gland epithelial cells(BMECs)were separated．from milk． (1)The expression of key enzymes of TG synthesis were examed by westem blot and the content of TG palmitic acid and DHA are detected by ELISA after inhibition or activation of mTOR singaling pathway in BMECs，which cells treated with mTOR inhibitior rapamycin or transfected with RhebRNA interference vector and Rheb overexpression vector．(2)The content change of TG was detected by ELISA and 37 kinds of fatty acid were examed by GC-MS after BMECs stimulated with insulin．(3) The content change of TG was detected