深海玫瑰杆菌固碳及蛋白质组分析海洋生物专业论文.docxVIP

目录2．5结果与讨论 目录 2．5结果与讨论 28 2．5．1生理培养及生理生化特征测定 28 2．5．2基于iTRAQ定量的比较蛋白组学分析 ．45 第三章一株来自于中国南海的玫瑰杆菌的分离与鉴定 53 3．1研究背景 ．．53 3．2材料与方法 ．53 3．3实验结果与讨论 ．59 3．4鉴定总结 ．．70 3．5本章节小结 ．．71 第四章本论文的主要结论和创新点 ．73 4．1主要结论 73 4．2创新点 ．73 4．3本论文的不足与展望 74 参考文献 75 硕士期间已发表的论文 ．86 致谢 87 万方数据 ContentSCoritents ContentS Coritents Abstract in Chinese ．I Abstract in English ．． ．． ．． ．． ．．． ． ．． ．II Chapter 1 Introduction ．．1 1．1 Marine microorganisms and marine carbon cycle ．．1 】【．2 study progresses ofRoseobacter Clade ． ． ．2 1．2．1 The introduction and distribution ofRoseobacter Clade ．2 1．2．2 The physiological and metabolic characteristics ofRoseobacter C lade 4 1．3 Trophic modes ofRoseobacter dade and the mixotrophic strategy ．．4 1．3．1 Trophic modes of Roseobacter clade ．．4 1．3．2 The mixotrophic strategy ofmarine microorganisms ．6 1．4 Carbon fixation pathways and the role ofRoseobacter Clade in carbon fixation．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．9 1．4．1 Different carbon fixation pathways of marine micmorganisrm ．9 1．4．2 Marine microorganiSlIE and marine carbon fixation 11 1．4．3 The role ofRoseobacter clade in carbon fixation ．．12 1 5 Marine sulfur cycle and the role of Roseobacter Clade in marine sulfur cycle ．．．． ．． ． ． ． ．．． ．14 1．5．1 Sulfur-oxidizing bacterias and marine sulfur cycle 14 1．5．2 The role ofRoseobacter clade in marine sulfur cycle ．．16 】I．6 Targets ofthis study and research significance ． ． ． ．．17 Chapter 2 Studies on the difference metabolic strategies of a deep-sea Roseobacter strain．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．18 2．1 Research background ．．18 2．2 Experimental design ． ． ． ．18 2．3 Experimental strain and culture medium ．．18 2．3．1 Experimental strain 18 2．3．2 Culture medium ． ．．18 2．4 Experimental methods． ． ． ． ． ． ．20 2．4．1 Foundamental culture 20 2．4．2 Physiological experiments ．． ．．．． ．． ． ． ． ． ． ．20 2．4．3 iTRAQ-based quantitive proteo