以cdse和cdznse量子点为标记物的电化学发光免疫分析分析化学专业论文.docxVIP

2．5参考文献 2．5参考文献 34 第三章以CdZnSe量子点为标记物的光谱型ECL免疫分析 ．37 3．1引言 37 3．2实验部分 38 3．2．1实验仪器 一 ．．38 3．2．2材料与试剂 38 3．2．3实验步骤 39 3-3结果与讨论 一41 3．3．1 CdZnSe、ZnSe NCs和Ab2[CdZnSe NCs的性质 41 3．3．2 CdZnSe NCs的形貌和结构 ．44 3．3．3 CdZnSe、ZnSe NCs和Ab2INCs的ECL行为 ．46 3．3．4光谱型ECL免疫传感器的电化学和ECL行为 ．48 3．3．5光谱型ECL免疫传感器的性能 49 3．4结论 ．53 3．5参考文献 53 致谢 ．．57 攻读硕士学位期间的科研成果 ．．58 II 万方数据 CONTENTSABSTRACT(Chinese)．． CONTENTS ABSTRACT(Chinese)．． ．．． ．．． ．．．．．． ．． ．． ．．．．．． ．．． ． ．． ．．．． ．．． ．．． ．．． ．． ． ．． ．．．I ABSTRACT III Chapter 1 Introduction ． ．．1 1．1 Introduction ． ．．1 1．2 Brief Introduction of ECL ．1 1．2．1 Concept of ECL ．： ．1 1．2．2 Character ofECL ． ． ．．2 1．2．3 Main materials to produce ECL ．2 1．2．4 ECL imaging technology ¨ ．．3 1．3 Brief Introduction of ECL Based on NCs 5 1．3．1 Models ofECL Based onNCs 5 1．3．2 Mechanisms ofECL Based on NCs ．．7 1．3．3 Applications of ECL Based on NCs ．8 1．4 Development Tendency ofECL Based on NCs．．．．．． ．．．．．．． ．．．．． ．． ．． ．．12 1．5 Background and Research Contents ofThe Dissertation 13 1．6 References 14 Chapter 2 ECL immunoassay with dual—stabilizers-capped CdSe NCs as Labels．．． ．20 2．1 Introduction ．20 2．2 Experimental Section 20 2．2．1 Apparatus ．．20 2．2．2 Reagents and Materials ．21 2．2．3 Experimemal procedure 22 2．3 Results and Discussion ．j ．．24 2．3．1 Optical Properties of CdSe NCs and Ab2INCs Conjugate ．．24 2。3．2 Electrochemistry ofthe Proposed ECL Immunosensor 25 2．3．3 ECL Performance ofthe Proposed ECL Immunosensor ．．27 2．3．4 Performance ofthe Proposed ECL Immunosensor ．．29 2．3．5 Th