冬小麦穗分化中生理指标变化及开花基因的差异表达分析作物专业论文.docxVIP

C0心兀卫》兀SCONTENTS C0心兀卫》兀S CONTENTS Abstract in Chinese ． I Abstract iIl English ． ． ． ． ．．III 1 IntroductiOII 1 1．1 Research progress ofwheat spike differentiation 1 1．1．1 Period of spike differentiation in Wheat ．1 1．1．2 Effects of temperature and photoperiod spike differentiation of Wheat ．2 1．1．3 Effects ofwater the spike differentiation ofWheat ．3 1．1．4 Effects of sowing date and ecological region the spike differentiation of Wheat ．．4 1．1．5 Effects of sugar differentiation ofⅥ，1leat ．4 1．1．6 Effects of nitrogen spike differentiation ofⅥ，lteat ．．5 1．2 Research progress of flowering related gene ． ． ． ．6 1．2．1 Plants ofthe FTfamily ．．． ． ． 6 1．2．2 Flowering related genes in Wheat ．． ． ． ． ． ． 7 1．2．3 The mode ofaction offlowering gene ． ． ． ．8 1．3 Research progress of root activity ．．8 1．4 Relationship between spike differentiation and yield and maturity stage in Wheat．．．．．．．．．．．．．．1 0 1．5 Purpose and significance ．1 1 2 Materials and Methods ．．13 2．1 T色st materials and design ．1 3 2．1．1’rest materials 13 2．1．2 T色st design ．1 3 2．2 Field sampling and processing method ．1 3 2．2．1 Investigation of spike differentiation ．1 3 2．2．2 Determination of sugar ．? ．13 2．2．3 Determination of soluble protein content 14 2．2．4 Determination ofroot activity ．．1zl 2．2．5 Calculation ofyield 15 2．3 Expression analysis of flowering related genes in the process of spike differentiation ．．1 5 2．：I．1 Sampling 15 2．3．2 Extraction ofRNA ．15 2．3．3 Reverse transcription of RNA ．．1 6 2．：；．4 Quantitative fluorescence primer 16 2．3．5 Gradient PCR and determination of annealing temperature 1 7 2．3．6 Fluorescence quantitative PCR reaction ．．1 8 2．4 Testing instruments and reagents ． l 8 2．4。l Tt：st instrument 1 8 m 万方数据 东北农业大学农业推广硕士学位论文2．4．2 东北农业大学农业推广硕士学位论文 2．4．2 Test reagent ． ． ． ． ． ． ． ． ． ． ． ． ． ． 19 2．5 Test data processing ．．19 3 Results and Analysis ．． ．．：!() 3．1 Analysis and comparison ofthe spike differe