利用微卫星标记对四带无须鲃不同种群的遗传质量评价陈晓虹张新铖.DOCVIP

利用微卫星标记对四带无须鲃不同种群的遗传质量评价 陈晓虹，张新铖，刘春，王芳，李凯彬【基金项目】 【基金项目】国家科技支撑计划（2015BAI09B05） Funded by National Science and Technology Support Plan of China（2015BAI09B05） 【作者简介】陈晓虹（1993-），女，本科，研究方向：修饰品系筛选和感染模型，E-mail：iris-chan0316@ 【通讯作者】李凯彬（1973-），男，研究员，E-mail：likaibins@126.com （中国水产科学研究院珠江水产研究所，广东广州 510380） 【摘要】 目的 分析四带无须鲃封闭群及近交群的遗传质量。 方法 筛选多态性丰富的四带无须鲃微卫星序列，通过构建两个多重PCR反应体系再利用毛细管电泳技术进行分型，开展群体遗传多样性分析。 结果 野生群、WT封闭群、BT封闭群及近交群的平均等位基因数分别为6.5833、3.1667、3.0833和3.1818，平均多态信息含量分别为0.5969、0.3748、0.4159和0.4241。群体遗传质量检测分析表明：4个群体之间遗传分化结果和近交群由野生群、封闭群逐渐筛选获得的过程相符。结论 筛选的微卫星标记可用于四带无须鲃不同群体的遗传质量分析，为其遗传质量控制及监测提供方法基础。 【关键词】四带无须鲃；微卫星标记；遗传质量；多重PCR Genetic quality evaluation in different populations of tiger barb, Puntius tetrazona used microsatellite markers CHEN Xiaohong，ZHANG Xincheng，LIU Chun，WANG Fang，LI Kaibin (Pearl River Fisheries Research Institute，CAFS．Guangzhou，510380，China) Corresponding author：LI Kaibin，E-mail：likaibins@126.com [Abstract] Objective To analyze and evaluate the genetic quality of closed populations and inbred population of tiger barb，Puntius tetrazona．Methods The microsatellite sequences with abundant polymorphism in tiger barb were screened，and two multiple PCR reaction systems were established．Then four populations were analyzed by capillary electrophoresis to identified the genotype and evaluated the genetic diversity．Results The average number of alleles in wild group，WT closed group，BT closed group，and inbred group were 6.5833，3.1667，3.0833，and 3.1818．The average polymorphic information content were 0.5969，0.3748，0.4159 and 0.4241，respectively．The analysis of population genetic quality showed that the results of genetic differentiation between the four groups were consistent with the process of the inbred population bred from the wild and closed group gradually．Conclusions These microsatellite markers can be used to analyze the genetic quality of different populations of tiger barb，providing a methodological basis for their genetic quality control and monitoring． [Key word