蛋白质晶体学课件.pdfVIP

第三章 蛋白质晶体学 1. 蛋白质的结晶和晶体生长 [主要内容]：蛋白质结晶的原理、技术和方法、对材料的要求、 晶体生长的理化条件、晶体的鉴定。 2. 晶体学基本概念 3. 衍射数据的收集 [主要内容]：晶体的收集与冷冻处理；X射线源的选择；衍射线记 录装置及其使用方法；衍射数据收集的全自动化。 4. 蛋白质结晶结构的解析 [主要内容]：蛋白质晶体空间群(Space group); 相位(Phase)的测 定；电子密度图的解释；结构模型的修正与精化。 5. pdb数据库文件以及结构的三维显示 Rapid Growth of X-ray Crystallography 1. Recombinant DNA 2. More powerful X-rays = Smaller crystals 3. Automated detectors 4. Fast cheap computers 5. Better computing algorithms and program suites 6. More systematic methods for growing crystals 7. Crystal cryopreservation 8. New approaches to the phase problem Section One Crystallization of Protein Make crystals of your protein 0.1-1.0mm in size Proteins must be in an ordered, repeating pattern. Protein crystallography workflow protein crystal expression purification crystallization data collection phasing structure solution General References: 1. Preparation and Analysis of Protein Crystals, A. McPherson (Robert E. Krieger Co, 1989) 2. Principles of Protein X-Ray Crystallography, J. Drenth (Springer, 1999) 3. Macromolecular Crystallography (Part A,B,C and D) Methods in Enzymology Vol. 276/277/368/374.(Academic Press, 1997 and 2003) Web Sites: 1. Crystal Growing 101: /support/support.html 2. Crystallography 101: /Xray/101index.html 3. Structural Medicine Course: http://www-structmed.cimr.cam.ac.uk/course.html 4. pyMOL: 5. Chime: /microbio/chime/ 6. Explorer: /microbio/chime/explorer/ 7. Swiss-PDB Viewer: /spdbv/ 8. Brookhaven Protein Data Bank: /pdb/ Electron Density (Structure) Crystallographer Computers