猪用抗千生素的替代.ppt

Probiotics益生素 悉生动物（已知菌动物） Targeting distribution 目的细菌在肠道中的定位分布情况，其与肠道菌系之 间的关系 Specific passway and function 具体作用途径与功能的研究 Development of antibiotics-free fermented feed无抗发酵饲料的开发 Experimental design试验设计 Bacteriophage 产生抗菌素 挥发性脂肪酸：乙酸、丙酸、丁酸 Tolerance of the intestinal environment 耐受肠道内环境 ZnO氧化锌 Hypothesis假设 Effects of dietary supplement of 3000 mg/kg ZnO on the expression of fundic gland Ghrelin gene in piglets日粮中添加3000 mg/kg氧化锌对断奶仔猪胃底腺Ghrelin基因表达的影响 Histamine concentrations in the small intestine of weanling piglets fed diets containing normal and high-levels of ZnO高锌日粮对断奶仔猪小肠组胺浓度的影响  The effects of dietary zinc on the changes in Isc (ΔIscZn) and in G (ΔGZn) 日粮锌对短路电流和电导率改变的影响 Enzyme preparations酶制剂 Herbal extracts(Astragalus polysaccharide, APS)天然提取物（黄芪多糖） Hypothesis 假设 Effects of dietary supplement of APS on serum cytokines in weaned pigs received cyclophosphamide (CY) APS（注射环磷酰胺, CY）对断奶仔猪血清细胞因子的影响 (pg/mL) Effects of APS on NO and iNOS activity in piglets APS对仔猪NO和iNOS的影响 Effects of APS on different cytokines APS对不同细胞因子的影响 Antimicrobial peptides(AMPs)抗菌肽 AMPs抗菌肽 Abundant and diverse groups of molecules that are encoded by definite genes 特定编码基因产生的一类小分子多肽 Important components of the innate immune system 先天免疫系统的重要组成成分 Current situation and existing problems现状及存在问题 Research of higher structure and antibacterial mechanism of AMPs 研究抗菌肽高级结构和抗菌机理 Short half-life, easy to lose activity 半衰期短，易失活 Toxicity toward prokaryotic cell 抗菌肽对原核细胞的毒性 Low yield, difficult to purify 天然抗菌肽纯化难度大，产量低 Modify the molecule of AMPs 优化抗菌肽分子 Select definite strain, and fusion expression 原核系统中选育特定抗性菌株，真核系统中采用融合 表达以提高其表达率 Special induction mode, reduce product cost 以特殊的诱导模式，降低生产成本 Molecule designing: IFN-γ gene + ABPS1 独特的分子设计: γ-干扰素基因与抗菌肽ABPS1序列串连 Two expression systems 采用两套表达系统，原核及真核表达，择优进行后续试验 Conditions for high expression efficiency 摸索提高表达效率的条件 Comp. Biochem. Physiol., Z. K. Feng, 2006 Zn improves