弓形虫Prugniaud株感染诱导小鼠抑制黑色素瘤效应的实验研究-临床检验诊断学专业论文.docxVIP

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弓形虫Prugniaud株感染诱导小鼠抑制黑色素瘤效应的实验研究-临床检验诊断学专业论文.docx

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弓形虫Prugniaud株感染诱导小鼠抑制黑色素瘤效应的实验研究-临床检验诊断学专业论文

PAGE PAGE 1 分类号 R446.69 密级学校代码 1 0 3 6 7 U D C 577 编号学号 20066831055 学位论文弓形虫 Prugniaud 株感染诱导小鼠黑色素瘤抑制作用的实验研究 Experimental study on the effect of Toxoplasma gondii Prugniaud strain infection-induced resistance to mice malignant melanoma 程启媛指导教师姓名孙新教授蚌埠医学院病原生物学教研室申请学位级别硕士学科专业临床检验诊断学论文提交日期 2009 年 4 月论文答辩时间 2009 年 5 月学位授予单位蚌埠医学院学位授予日期 2009 年 6 月答辩委员会主席：论文评阅人： 2009 年 4 月目录中文摘要·····················································································2 英文摘要·····················································································4 前言 ·························································································7 材料和仪器 ·············································································· 10 实验方法··················································································· 12 实验结果··················································································· 22 讨论 ······················································································· 32 结论 ······················································································· 35 参考文献··················································································· 36 致谢 ······················································································· 39 附录 A 中英文术语及缩略词对照表 ····················································· 40 附录 B 常用试剂配制 ······································································· 42 附录 C 个人简历及发表论文 ······························································· 44 附录 D 综述 ··················································································· 45 弓形虫 Prugniaud 株感染诱导小鼠抑制黑色素瘤效应的实验研究摘要目的：检测弓形虫（Toxoplasma gondii, TG）Prugniaud 株单独或联合卡介苗（BCG）作用后对小鼠恶性黑色素瘤的抑制作用效果，并尝试从不同侧面探讨其作用机制，为筛选可能具有诱发非特异性免疫保护作用的组分提供新的线索。方法：将绿色荧光标记的转基因小鼠 GFP C57/B6 接种 B16 细胞，建立荷瘤模型，将荷瘤小鼠随机分为 4 组，每组 9 只；两天后，TG/BCG/B16 组腹腔接种弓形虫 Prugniaud 株（TG）和卡介苗（BCG），TG/B16 组接种 Prugniaud 株，