复方甘草酸苷对溃疡性结肠炎大鼠Toll样受体介导的NF-κB信号通路的影响-中西医结合临床专业论文.docxVIP

I I 摘 要 目的：探讨复方甘草酸苷对溃疡性结肠炎大鼠Toll样受体介导的核因子 -κB（NF-κB）信号通路的影响。 方法：健康SD大鼠49只取10只作为正常对照组（A组），其余39只大鼠采 用TNBS/乙醇联合建模法制备溃疡性结肠炎大鼠模型，并随机分为模型对 照组（B组），柳氮磺吡啶组（C组）、复方甘草酸苷组（D组），分别给 予生理盐水、柳氮磺吡啶与复方甘草酸苷干预处理。 结果：1.肉眼与病理学观察发现大鼠溃疡性结肠炎模型是成功的。观察大 鼠模型制作前后及干预治疗后的毛发、粪便性状、便血和体重是有较大的 变化的。但是对比干预治疗的两组柳氮磺吡啶组（C组）和复方甘草酸苷 组（D组）大鼠的毛发、粪便性状、便血和体重改变之间变化不大。 2.复方甘草酸苷组的血清TLR4与NF-κB p65表达量明与柳氮磺吡啶组和 正常对照组对比无明显差异，但显低于模型对照组(P 0.05)。 3.复方甘草酸苷组NF-κB p65与TLR4蛋白阳性表达率与柳氮磺吡啶组和 正常对照组对比无明显差异，但明显低于模型对照组(P 0.05)。 4.Pearson相关分析显示NF-κB p65与TLR4在溃疡性结肠炎模型中的表达 呈正相关关系(P 0.05)。 结论：复方甘草酸苷治疗组大鼠血清中TLR与NF-κB p65水平对比模型对 照组减少，对比柳氮磺吡啶组无明显差异；结肠组织蛋白阳性达率明显低 于模型对照组，对比柳氮磺吡啶组无明显差异，说明复方甘草酸苷阻断 Toll样受体介导的NF-κB信号通路。 Toll样受体介导的NF-κB信号通路在炎症性肠病的发生发展中起重要作 用，复方甘草酸苷阻断该通路中TLR与NF-κB p65的表达，进而为临床用 药提供理论依据。 关键词 溃疡性结肠炎；Toll样受体；NF-κB信号通路；复方甘草酸苷 II II Compound glycyrrhizin glucoside toll-like receptor mediated on ulcerative colitis rats The influence of the nf-kappa B signaling pathway Speciality: Integrative Medicine Digestive Diseases direction Author:Lan Xiangyang Tutor:Professor Lu Guangxin Abstract Objective: To investigate the Toll-like receptor-mediated NF -κB signaling pathway effect of glycyrrhizin for Ulcerative Colitis. Methods: Selected 49 healthy SD rats, the 10 rats were as the normal control group (group A), and the remaining 39 rats were combined modeling TNBS / ethanol prepare legal ulcerative colitis and then were randomly equally divided into model group (group B), sulfasalazine group (group C), glycyrrhizin group (group D) that were given saline, sulfasalazine and glycyrrhizin intervention treatment. Results: 1.The naked and pathology observed the ulcerative colitis in rats with colorectal cancer model was successful. Were observed before and after rat model and intervention treatment after the hair, feces traits, bloody and weight is a bigger change. But contrast intervention treatment of two groups of willow nitrogen sulfonyl pyridine group (group C) and compound glycyrrhizin glucoside group (group D) rats between hair, feces trai