地黄饮子对aβ致海马神经元损伤影响的实验研究-中医基础理论专业论文.docxVIP

黑龙江中医药入学博十学位论文ABSTRACT 黑龙江中医药入学博十学位论文 ABSTRACT On creating model of cultured hippocampal neurons injured by Bamyloid，we studied the mechanisms of close interrelation betweenBamyloid and Alzheimer’s disease at cell and molecular level with advanced test methods．For example，the technique of optical 1ens alld electroll microscope、 immunohistochemical method、biochemistry、molecular biology and HPLC，so as to deeply probe into the mechanisms that Decotion of Rehamanniae pretects and treats Alzheimer’s disease．The results show：1、Decotion of Rehamanniae can significantlY ame】iorate the existent state of hippocampal neu rons and increase the activitY of neuroas．2、 Decotion of Rehamanniae can effectively reduce the excessive response of Immediate—Early-Gene c—fos and c-iun when 13amyloid injurs hippocampal neurons．and increase the stress response of hippocampal nen rons to exoteric harmful stimulation．3、Decotion of Rehamanniae can inerease the activitY of antioxidant enzyme SOD、CAT、and GSH．PX in the caltured fluid，and improve the cleanup abilitv of free tadicals．4、Decotion of Rehamanniae can effectively control over the overloading of caleium thatl3amyloid injured hippocampal neurons，and protect the frame of membrane． 5、Decotion of Rehamanniae can repress the excessive apoptosis of hippocampal neurons injured by Bamyloid through modulating the ratio of bcl．2／bax and reduee the exPression of casPase．3 gene．6、 ExtracorDoral experimental research shows：Decotion of Rehamanniae can dose-dependly protect hippocampal neurons and the best effect is the high dose’s．And this research also shows that： the curative effect of Decotion of Rehamanniae on hippocampal neurons carries out by two approaches：1、By penetrating blood．brain barrier the effective components of Decotion of Rehamanniae protect hippocampal neurons from injury．2、 Decotion of Rehamanniae